How do we perform GM-CSF lab test?
Protocols we follow to perform GM-CSF lab assays
We, at NorthEast BioLabs, use GM-CSF ELISA, MSD, and Luminex assay to detect granulocyte-macrophage colony-stimulating factor in your sample. We can detect GM-CSF protein in serum, plasma, and cell/tissue culture supernatant. Following is a brief Luminex protocol.
The assay to test the GM-CSF structure and its function begins with 80 μl of wash buffer in each well. We shake it for 10 mins and decant the wells. Once the residue is removed, we add standard, control or samples in the appropriate wells followed by assay buffer and beads to all wells and incubate the wells overnight at 4℃. After the bead captures an analyte from the sample, we introduce a biotinylated detection antibody in each well and incubate them for 1 hr at RT. Post incubation, 20 μl of streptavidin-phycoerythrin, a reporter molecule, and incubated for 30 mins at RT. We then wash the wells with a wash buffer, add 70 μl of drive or sheath fluid and read the plate on a suitable Luminex platform.
The GM CSF Luminex assay help to quantify fluorescent reporter signals. The assay is highly sensitive, with an inter-assay precision of < 20.8% and intra-assay precision of < 2.7%. The assay is also accurate, with an accuracy of 94% recovery in serum matrix.