Brain-Derived Neurotrophic Factor (BDNF)

Brain-Derived Neurotrophic Factor (BDNF) is a protein encoded by the BDNF gene in humans. It supports the survival of neurons in the central and peripheral nervous systems. BDNF functions as a neurotransmitter modulator and engages in neuronal plasticity. BDNF protein encourages the growth and maturation (differentiation) of new neurons and synapses. The hippocampus, cortex, and basal forebrain responsible for learning, memory, and higher thinking, actively host the BDNF biomarker. The kidneys, retina, motor neurons, saliva, and skeletal muscle are also homes for the BDNF protein. When searching for ‘What is BDNF’, you can also search for its aliases – Abreneurin, ANON2, and BULN2.

BDNF: Neurobiology, Neurodegeneration, and Metabolic Biomarker

Brain-Derived Neurotrophic factor is vital for long-term memory. The brain retains the capability to grow neurons from stem cells, even as most human neurons have prenatal formation. Neurogenesis is this process of stimulated and controlled neural growth for the development, maintenance, and function of vertebrate nervous systems for improved cognitive function. BDNF biomarker is one of the most active neutrophils, which are proteins that aid the stimulation of neurogenesis. Other researched BDNF functions and effects include synaptic plasticity, inflammation and immunity, growth of cardiac and endothelial cells, lipid metabolism, etc. Specific exercises, such as treadmill in mice and aerobics in humans, are considered BDNF growth factor.

BDNF Assay: Established, Multi-species MSD, Luminex, and ELISA Assay

BDNF level considered normal in humans and other species is an area of active biological research. At NorthEast BioLab, we tailor BDNF testing to suit your desired assay parameters through BDNF MSD, Luminex, and ELISA. Typically, these BDNF assays employ the fundamentals of the quantitative sandwich enzyme immunoassay technique explained below.
First, we pre-coat the antibody specific to capture BDNF biomarker onto a microplate and follow up by a plate wash to remove any unbound substances. Next, calibration standards, quality controls, and biological samples get pipetted into the plate wells, and the immobilized capture antibody binds any BDNF present. Then, we wash off any unbound material following proper shaking and incubation as per method protocol. Later, a biotin-conjugated antibody specific for BDNF detection gets added to the wells· After washing, we add avidin conjugated Horseradish Peroxidase (HRP) to the plate wells and rinse again to remove any unbound avidin-enzyme reagent. Eventually, we add a substrate solution to the plate wells, and color develops in proportion to the amount of BDNF bound in the initial step. The final step in BDNF ELISA requires us to halt the color development and quantitate the color intensity with a suitable plate reader.

Over the years, NorthEast Biolab has acquired the experience to reproduce high-quality BDNF assays. Our scientists and researchers can perform BDNF assay to measure the analyte in multiple formats. We have extensive expertise on BDNF assay specifics, such as matrix type, tissue lysis, volume requirements, sample dilution, and multiplexing possibilities. Our scientists rely only on preapproved vendors and recommended kits, antibodies, diluents, buffers, substrates, reagents, calibrators, control matrices, etc. for running BDNF Luminex and MSD Assay. We follow the industry best practices to ensure accuracy and test your biomarkers through high-affinity materials. Our focus on efficiency and operational excellence helps us deliver high-quality BDNF testing services to all our sponsors.

Drug Discovery and Development Process

How long do you think it takes for a new drug to get approved in the US? About 12-15 years, that’s roughly 1/3rd duration of the entire professional career for any of us. Next, how much investment do you think is required to bring a new drug to market?

Popular Brain derived Neurotrophic Factor (BDNF) Resources

Numerous publications have associated BDNF biomarker levels with conditions affecting various brain functions. Some of these include depression, schizophrenia, obsessive-compulsive disorder, Alzheimer’s disease, Huntington’s disease, Rett syndrome, dementia, and anorexia nervosa and bulimia nervosa neurodegeneration. Increasingly, literature illustrates the considerable interest in the presence of brain-derived neurotrophic factor (BDNF) in human blood.

Here are some resources for you to read further on the BDNF protein:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504526/
https://ghr.nlm.nih.gov/gene/BDNF
https://elifesciences.org/articles/15092/
https://www.sciencedirect.com/topics/medicine-and-dentistry/brain-derived-neurotrophic-factor/