Carryover

  • During method validation runs, a double-blank sample should be injected after the highest calibration standard to determine if the equipment is cross-contaminating the sample during loading.
  • Peak response for the analyte(s) due to carryover must not be more than a specified % of the response of the lowest calibration standard (LLOQ). For internal standard again, the response must not be more than a determined % of the average internal standard response.