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    Click below for answers to your LI-COR Odyssey Western Blot Analysis questions -

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    Western Blot Analysis using LI-COR Odyssey relies on the equipment normalizing signal intensities from two IR detection channels to deliver protein quantitation with comparable sensitivity to chemiluminescence. LI-COR Odyssey relies on longer wavelengths from near-infrared imaging for detecting strong and weak bands on a western blot to produce a low signal-to-noise ratio as well as a wide and linear dynamic range. NorthEast BioLab effectively applies LI-COR Odyssey to offer precise, rapid, and robust Western Blot Analysis, In-Cell Western, and Protein Gel Imaging.

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    What is LI-COR Odyssey Western Blot Analysis?

    LI-COR Odyssey For High-Sensitivity Western Blot Analysis

    LI-COR Odyssey is a flexible, IR laser-based instrument that offers enhanced data quality and sustained sensitivity and can readily detect even subtle mobility shifts due to protein modifications. It uses infrared fluorescent (IR fluorescent) antibody conjugates to identify strong and weak bands on a western blot. LI-COR adopts a simultaneous ratiometric approach with its 700 and 800 nm infrared (IR) fluorescent detection channels that facilitate a two-color target analysis and normalization.

    The platform facilitates protein quantification in a wide linear range and offers flexibility for the blot processing and storage as the signal from the IR fluorescent dyes is robust. LI-COR Odyssey facilitates both chemiluminescent and IR fluorescent protein detection without film usage. This elimination allows flexibility, data archiving, darkroom and developer cost savings, and environmental friendliness.

    Key Li-COR benefits include superior sensitivity, quantification, and linear range –

    • LI-COR Odyssey near-IR protein quantification discern strong and weak bands on a western blot accurately because fluorescent detection is static, unlike the dynamic light generation in chemiluminescence
    • The longer wavelengths of near-IR fluorescent detection result in reduced autofluorescence of membrane surfaces and biomolecules, resulting in lower signal-to-noise ratio and increased sensitivity with NIR fluorophores
    • NIR allows a broader, linear detection range such that protein concentrations falling within the detection limits of the instrument are visible in the scanned image
    • LI-COR Odyssey compares well in chemiluminescence scenarios, wherein optimization of exposure times for high protein concentrations leads to undetectable/unquantifiable bands for lower protein concentrations and vice-versa

    Sponsors Love Our Bioanalytical Laboratory Services For Top Quality And Industry Leading Turnaround

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    NorthEast BioLab is by far the most responsive and thorough bioanalysis lab services CRO.

    We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.

    We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects.

    We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data.

    We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

    NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism.

    NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

    NorthEast BioLab truly goes that extra mile, and we hope to continue enjoying seminal interactions with them.

    NorthEast BioLab presents a science-based, hands-on, no-frills approach on the latest bioanalytical platforms.

    We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

    We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

    Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

    NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

    Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

    This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with top quality and reporting standard with incredible responsiveness.

    As a small company, our vendors are key members of our project teams. The scientists at NorthEast BioLab are technical experts, who produce high-quality data, on-time, and on-budget.

    NorthEast BioLab is by far the most responsive and thorough bioanalysis lab services CRO.

    We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.

    We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects.

    We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data.

    We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

    NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism.

    NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

    NorthEast BioLab truly goes that extra mile, and we hope to continue enjoying seminal interactions with them.

    NorthEast BioLab presents a science-based, hands-on, no-frills approach on the latest bioanalytical platforms.

    We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

    We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

    Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

    NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

    Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

    This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with top quality and reporting standard with incredible responsiveness.

    As a small company, our vendors are key members of our project teams. The scientists at NorthEast BioLab are technical experts, who produce high-quality data, on-time, and on-budget.

    Tackle Your Western Blot Analysis With Our Veteran Team Now!

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    Which Li-COR Odyssey Applications do we Offer?

    Li-COR Odyssey Assay: Western Blot Analysis, In-Cell Western, and Protein Gel Imaging

    In the LI-COR Odyssey infrared imaging system, the sensitive two-color near IR fluorescence detection allows simultaneous total and phosphorylated protein quantitation without stripping and reprobing the blots. We can also use Li-COR for gel imaging or in-gel Westerns for proteins that are too small, too large, or do not transfer well to membranes. Another assay where we can use LI-COR is the in-cell western used to detect and quantify proteins in cells fixed to microplates. Here, we follow the same steps as in western blotting except that lysate preparation, quantification, electrophoresis, and membrane transfer get eliminated. In-cell western assays can be used to study cell signaling and G-protein coupled receptor function, internalization, and recycling in neurology and oncology.

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    Why Choose us for LI-COR Odyssey Western Blot Analysis?

    Rapid and Quality LI-COR Odyssey Western Blot Analysis

    Our scientists, NorthEast BioLab, develop reliable bioanalytical methods and perform your sample analysis assay for various stages of drug development. We handily carry out your protein quantification using western blots for pharmacodynamics studies in preclinical and clinical studies. We use LI-COR western blots to accurately detect cell proliferation, apoptosis, cell signaling, and various biomarkers, and quantitate proteins in a fuller, linear range as well as transfer images and data files to clients for convenient and safe long-term storage.

    The Basics of Bioanalysis

    Bioanalysis is an essential tool in drug discovery and development for determining the concentration of drugs and their metabolites as well as various pharmacodynamics biomarkers in biological fluids. In these analyses, scientists use developed an….

    How does Western Blot Analysis work on Li-COR Odyssey?

    Li-COR Odyssey: Near-Infrared Imaging for Western Blot Bands

    Here’s a comprehensive Li-COR Odyssey protocol with some steps relevant only to appropriate protein assays, such as western blots, in-gel westerns, or in-cell westerns

    • Perform polyacrylamide gel electrophoresis on protein samples
    • Transfer the proteins to a nitrocellulose or polyvinylidene membrane
    • Incubate membrane with primary antibodies and limit non-specific binding
    • Incubate Near-IR (NIR) fluorescent secondary antibodies with the membrane to bind these with the primary antibodies
    • Identify and quantify protein via ratiometric detection by the Li-COR Odyssey infrared imaging system after washing away unbound antibodies

    In the Li-COR Odyssey platform, we use one of the two IR detection channels (700 and 800 nm) for our protein of interest and the other channel for a housekeeping protein. Afterward, we achieve accurate quantitation by normalizing the signal intensities from the two proteins. The technology can also be used to bridge enhanced chemiluminescence (ECL) and IR fluorescence imaging. In this case, we use appropriate near-IR fluorescent antibodies directed against horseradish peroxidase (HRP)-conjugated antibodies already bound to either primary antibodies or immobilized target proteins.

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