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  • ELISA Method
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                                                        • Cell-based Assays

                                                        Cell-based Assays

                                                        Custom cell-based assay method development and optimization to test the potency or biological activity of your drug products

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                                                        Click below for answers to your Cell Based Assay Development questions -
                                                        • What are Cell-based Assays?
                                                          What are Cell-based Assays?
                                                        • What is the Role of Cell-based Assays during Drug Development?
                                                          What is the Role of Cell-based Assays during Drug Development?
                                                        • What are the various Cell-based Assays Offered by Us?
                                                          What are the various Cell-based Assays Offered by Us?
                                                        • Why Choose NorthEast BioLab for Your Cell Proliferation, Viability, and Toxicity Assays?
                                                          Why Choose NorthEast BioLab for Your Cell Proliferation, Viability, and Toxicity Assays?

                                                        Ask Us About Your Study

                                                        Custom cell-based assay method development and optimization to test the potency or biological activity of your drug products

                                                        SPEAK TO OUR SCIENTISTS

                                                        What are Cell-based Assays?

                                                        Cell-based Assays: Understanding Sub-cellular Localization and Pathways

                                                        Contrary to biochemical assays that analyze the cell lysate, Cell based assays measure different parameters of fixed or living cells. Cell based assays can be used to determine the growth rate of a cell population and the pathways and how they interact within a cell. These assays are used in various stages of drug discovery and development, due to their flexibility, adaptability, and versatility. For example, Cell based assays are commonly utilized in spatiotemporal analysis to understand the modifications that are associated with proliferating cells. We, at NorthEast BioLab, use Cell based assay development for sub-cellular localization and drug discovery screening in pharmaceutical development.

                                                        Cell based assay development is utilized to analyze the cytotoxicity of a chemical compound or observe its toxic impact on the human body. Since biotherapeutics have several functional domains that can interact with different molecules to achieve optimum functioning, Cell based assays are favored during the development of biologics. For example, a protein may have a functioning domain which stimulates cell internalization or activates a transcription factor. This makes it necessary to test these domains and related functions of the drug compound before product release. In the final stages of the study, these functional domains may be tested on a living organism, animal, or human volunteer. Initially, however, the biological activity is tested through Cell based assays.

                                                        What Our Sponsors Have to Say?

                                                        VP, Bioanalytical Development

                                                        NorthEast BioLab is by far the most responsive and thorough bioanalysis lab services CRO.

                                                        VP, Biomarker Development 

                                                        We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.

                                                        Executive Director, Pharmacokinetics

                                                        We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects.

                                                        President & CSO, Biotech

                                                        We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data.

                                                        Sr. Associate Director, Clinical Trials

                                                        We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

                                                        Co-Founder, Biotech & University PI

                                                        NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism.

                                                        VP, PK PD Analysis

                                                        NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

                                                        Head, Bioanalytical Development

                                                        NorthEast BioLab truly goes that extra mile, and we hope to continue enjoying seminal interactions with them.

                                                        Sr.Director, Bioanalytical Development & QC

                                                        NorthEast BioLab presents a science-based, hands-on, no-frills approach on the latest bioanalytical platforms.

                                                        SVP Quality & Compliance, Biotech

                                                        We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

                                                        Director, PK

                                                        We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

                                                        VP, Head of Clinical Operations

                                                        Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

                                                        Executive Director, Drug Research

                                                        NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

                                                        Director, Bioequivalence Testing

                                                        Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

                                                        Head, PK

                                                        This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with top quality and reporting standard with incredible responsiveness.

                                                        VP, Development Operations

                                                        As a small company, our vendors are key members of our project teams. The scientists at NorthEast BioLab are technical experts, who produce high-quality data, on-time, and on-budget.

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                                                        Consult with a Scientist About Your Cell Based Assay Study
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                                                        What is the Role of Cell-based Assays during Drug Development?

                                                        Cell-based Assays: From Drug Discovery and Preclinical to Clinical Phases

                                                        Cell based assays are extensively used in drug discovery and development, as this method provides feedback from experiments with living cells. These tests include various types of assays, some of which are already discussed above, such as toxicity assay, cell proliferation assay, motility assays, etc.

                                                        Cell based assays allow scientists to monitor cell behavior and achieve accurate quantitation of cellular pathways. Undoubtedly, the obtained insights from these experiments facilitate drug development across various phases such as discovery, preclinical, and clinical. Thus, Cell based assay development can be critical in quickly identifying potentially viable drug candidates while eliminating the need for multiple screening methods.

                                                        A few applications of Cell based assays in drug discovery and development are as under:

                                                        • Identifying antiviral and antimicrobial compounds
                                                        • Evaluating non-toxic and toxic drug substances
                                                        • Detecting viral contaminations harmful to the body
                                                        • Recognizing pathogen-associated molecular patterns (PAMPs)
                                                        • Screening pathogen recognition receptor antagonists and agonists

                                                        Cell based assays are used in measurements of various biological markers critical to drug discovery and development. At NorthEast BioLab, we implement Cell based assays using clinical and FDA-approved standards.

                                                        The Basic of Bioanalysis

                                                        The Basics of Bioanalysis

                                                        Bioanalysis is an essential tool in drug discovery and development for determining the concentration of drugs and their metabolites as well as various pharmacodynamics biomarkers in biological fluids. In these analyses, scientists use developed an…

                                                        Read More at our Learning Center

                                                        What are the various Cell-based Assays Offered by Us?

                                                        Cell-based Assays by NorthEast BioLab: Cell Proliferation, Viability, and Toxicity Assays

                                                        Cell-based assays are used to understand the processes occurring at the cellular levels, such as cell proliferation assays and viability assays that measure the growth and activity of cell populations. At NorthEast BioLab, we offer the three types of Cell based assays explained below.

                                                        Cell Proliferation Assay

                                                        Cell proliferation is a natural process which divides and increases the number of cells in the human body. This process helps in maintaining an equilibrium against cell-death for healthy body functioning. Therefore, typical cell proliferation rates are critical to the human body. In diseases such as cancer, cells may have varying cell proliferation rates.

                                                        Cell proliferation within the body can be measured using the metabolic activity of the cell population. At NorthEast BioLab, we use various dyes such as MTT and WST-1 that lead to cell permeabilization. Through an enzyme reaction, the end-product of the experiment is a color that forms the basis for the measurement of cell proliferation.

                                                        Often, our scientists use fluorescence dyes whenever high sensitivity is required for drug discovery and development. These dyes exhibit higher sensitivity when compared to the cell proliferation assay developed using colorimetric dyes. The fluorescent dye method is beneficial while comparing the small differences in the cell proliferation rates of two cell populations.

                                                        Cell Viability Assay

                                                        Cell viability assays are used to relate cell number and cell behavior in a population, offering a clear understanding of healthy cell structure, enzyme activity, and cellular metabolism. For example, using cell viability tests, scientists can measure the anabolic activity of the Cell.

                                                        Cell viability measurements play an essential role in drug discovery and development. In fact, in toxicity assay, measuring cell viability is the primary purpose of the experiment.

                                                        Various methods are used for cell viability assays such as TMRE, MTT, ATP luminescence, and calcein violet. The viable method is generally decided through several factors such as complexity, speed, and equipment cost.

                                                        Cell Toxicity Assay

                                                        Cell toxicity assays are used to assess the toxicity of drug compounds. When cells in the human body are exposed to cytotoxic compounds, they can undergo apoptosis, necrosis, or lose membrane integrity. Cell death may harm cell populations and various bodily functions. Hence, Drug candidates that are too toxic are eliminated in subsequent drug discovery phases. That said, if the drug aims at reducing select cell proliferation, cell toxicity may be desirable as in cancer therapeutics.

                                                        Registered And Inspected By Various Regulatory Agencies

                                                        Why Choose NorthEast BioLab for Your Cell Proliferation, Viability, and Toxicity Assays?

                                                        Expedited Drug Screening and Development using Cell Proliferation, Viability, and Toxicity Assays

                                                        NorthEast BioLab offers several Cell based assays used in different phases of drug discovery and development. From target validation to lead optimization, Cell based assays (including cell proliferation, cell viability, and cell toxicity assays) help achieve quick iterations in drug discovery and development. In addition to the identification of new drugs, effective Cell based assays are used to determine the potency, efficacy, safety, selectivity, and specificity of these drugs. Furthermore, our scientists ensure that your tailored research is conducted thoroughly, FDA-guidelines are followed attentively, the viability and toxicity of your drug compounds are assessed correctly. This attention to detail helps us deliver rapid and reliable results to our clients every time.

                                                        Still Have Questions Regarding Your Cell-based
                                                        Assays?

                                                        The Scientists At NorthEast BioLab are Ready to Help!
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                                                        Explore More Services

                                                        • Drug Metabolism and Pharmacokinetics (DMPK) Drug Metabolism and Pharmacokinetics (DMPK)
                                                        • ADME Studies ADME
                                                          Studies
                                                        • Dose Formulation Analysis Dose Formulation
                                                          Analysis
                                                        • Cytotoxicity Assay Cytotoxicity
                                                          Assay
                                                        • Biochemical Assay Biochemical
                                                          Assay
                                                        • Mechanism of Action (MOA) Studies Mechanism of Action (MOA) Studies

                                                        Expertise

                                                        • Liquid Chromatography Mass Spectrometry
                                                        • ELISA Method
                                                        • MSD Assay
                                                        • Luminex Multiplex Assay
                                                        • LI-COR Odyssey Assay
                                                        • PK PD Analysis
                                                        • Method Development Validation and Transfer

                                                        Services

                                                        • Pharmacokinetics (PK) Study
                                                        • Toxicology (Tox) Study
                                                        • Biomarker Testing Services
                                                        • Bioequivalence and Bioavailability Studies
                                                        • Immunogenicity Testing
                                                        • Bioanalytical Assay

                                                        Solutions

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                                                        • Clinical CRO
                                                        • Preclinical CRO
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                                                        • Non-GLP Testing

                                                        About Us

                                                        • Overview
                                                        • Why Us?
                                                        • Values
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                                                        • News and Events
                                                        • Testimonials
                                                        • Contact Us
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