What is the Role of Cell-based Assays during Drug Development?
Cell-based Assays: From Drug Discovery and Preclinical to Clinical Phases
Cell based assays are extensively used in drug discovery and development, as this method provides feedback from experiments with living cells. These tests include various types of assays, some of which are already discussed above, such as toxicity assay, cell proliferation assay, motility assays, etc.
Cell based assays allow scientists to monitor cell behavior and achieve accurate quantitation of cellular pathways. Undoubtedly, the obtained insights from these experiments facilitate drug development across various phases such as discovery, preclinical, and clinical. Thus, Cell based assay development can be critical in quickly identifying potentially viable drug candidates while eliminating the need for multiple screening methods.
Some of the common applications of cell-based assays in drug discovery and development are as under:
- Evaluating non-toxic and toxic drug substances that can affect cell viability, proliferation, differentiation, migration, or death
- Screening pathogen recognition receptor antagonists and agonists that can modulate immune responses and inflammation
- Recognizing pathogen-associated molecular patterns (PAMPs) that can trigger innate immune responses and inflammation
- Detecting viral contaminations that can harm the body or interfere with the quality of biopharmaceutical products
- Identifying antiviral and antimicrobial compounds that can inhibit or kill pathogens or infected cells
Cell based assays are used in measurements of various biological markers critical to drug discovery and development. At NorthEast BioLab, we offer a wide range of cell-based assay services for various types of studies. We use state-of-the-art bioanalytical technologies and platforms, such as ELISA, Meso Scale Discovery, Luminex, qPCR, dPCR, LC–MS, and Western Blotting, to develop and validate robust and reliable cell-based assays. We also follow GLP/GCLP standards and FDA guidelines to ensure the quality and compliance of our cell-based assays.