Western Blot Analysis using LI-COR Odyssey relies on the equipment normalizing signal intensities from two IR detection channels to deliver protein quantitation with comparable sensitivity to chemiluminescence. LI-COR Odyssey relies on longer wavelengths from near-infrared imaging for detecting strong and weak bands on a western blot to produce a low signal-to-noise ratio as well as a wide and linear dynamic range. NorthEast BioLab effectively applies LI-COR Odyssey to offer precise, rapid, and robust Western Blot Analysis, In-Cell Western, and Protein Gel Imaging.
LI-COR Odyssey is a flexible, IR laser-based instrument that offers enhanced data quality and sustained sensitivity and can readily detect even subtle mobility shifts due to protein modifications. It uses infrared fluorescent (IR fluorescent) antibody conjugates to identify strong and weak bands on a western blot. LI-COR adopts a simultaneous ratiometric approach with its 700 and 800 nm infrared (IR) fluorescent detection channels that facilitate a two-color target analysis and normalization.
The platform facilitates protein quantification in a wide linear range and offers flexibility for the blot processing and storage as the signal from the IR fluorescent dyes is robust. LI-COR Odyssey facilitates both chemiluminescent and IR fluorescent protein detection without film usage. This elimination allows flexibility, data archiving, darkroom and developer cost savings, and environmental friendliness.
Key Li-COR benefits include superior sensitivity, quantification, and linear range –
Our projects with NorthEast BioLab include method development, stability studies, cross-validation of other labs, support for investigator-sponsored studies, and Clinical Phase I – IV studies. They have considerably exceeded our expectations time and again.
NorthEast BioLab provided critical insight into data analysis, and are compliant with industry best practices and regulatory standards. We would certainly recommend them and look forward to working together in the future.
NorthEast Biolab truly goes that extra mile to make the collaboration successful, and I will continue to enjoy very productive interactions with them in the future. The integrity of the staff is as refreshing as their willingness to provide ingenious scientific input and high-quality data.
Our latest study together was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis. This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with very high quality and reporting standard and incredible responsiveness.
NorthEast Biolab is by far the most responsive and thorough bioanalysis service provider. They work closely with the client to provide the most efficient and cost-effective approach to get the job done.
We have worked with NorthEast Biolab on several programs and have found them to be professional, collaborative, and responsive. As a small company, our vendors are key members of our project teams. The scientists at NorthEast Biolab are technical experts, who produce high-quality data, on-time, and on-budget.
Here’s a comprehensive Li-COR Odyssey protocol with some steps relevant only to appropriate protein assays, such as western blots, in-gel westerns, or in-cell westerns
In the Li-COR Odyssey platform, we use one of the two IR detection channels (700 and 800 nm) for our protein of interest and the other channel for a housekeeping protein. Afterward, we achieve accurate quantitation by normalizing the signal intensities from the two proteins. The technology can also be used to bridge enhanced chemiluminescence (ECL) and IR fluorescence imaging. In this case, we use appropriate near-IR fluorescent antibodies directed against horseradish peroxidase (HRP)-conjugated antibodies already bound to either primary antibodies or immobilized target proteins.
Bioanalysis is an essential tool in drug discovery and development for determining the concentration of drugs and their metabolites as well as various pharmacodynamics biomarkers in biological fluids. In these analyses, scientists use developed an….
In the LI-COR Odyssey infrared imaging system, the sensitive two-color near IR fluorescence detection allows simultaneous total and phosphorylated protein quantitation without stripping and reprobing the blots. We can also use Li-COR for gel imaging or in-gel Westerns for proteins that are too small, too large, or do not transfer well to membranes. Another assay where we can use LI-COR is the in-cell western used to detect and quantify proteins in cells fixed to microplates. Here, we follow the same steps as in western blotting except that lysate preparation, quantification, electrophoresis, and membrane transfer get eliminated. In-cell western assays can be used to study cell signaling and G-protein coupled receptor function, internalization, and recycling in neurology and oncology.
Our scientists, NorthEast BioLab, develop reliable bioanalytical methods and perform your sample analysis assay for various stages of drug development. We handily carry out your protein quantification using western blots for pharmacodynamics studies in preclinical and clinical studies. We use LI-COR western blots to accurately detect cell proliferation, apoptosis, cell signaling, and various biomarkers, and quantitate proteins in a fuller, linear range as well as transfer images and data files to clients for convenient and safe long-term storage.
