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Western Blot Analysis using LI-COR Odyssey relies on the equipment normalizing signal intensities from two IR detection channels to deliver protein quantitation with comparable sensitivity to chemiluminescence. LI-COR Odyssey relies on longer wavelengths from near-infrared imaging for detecting strong and weak bands on a western blot to produce a low signal-to-noise ratio as well as a wide and linear dynamic range. NorthEast BioLab effectively applies LI-COR Odyssey to offer precise, rapid, and robust Western Blot Analysis, In-Cell Western, and Protein Gel Imaging.
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What is LI-COR Odyssey Western Blot Analysis?
LI-COR Odyssey For High-Sensitivity Western Blot Analysis
LI-COR Odyssey is a flexible, IR laser-based instrument that offers enhanced data quality and sustained sensitivity and can readily detect even subtle mobility shifts due to protein modifications. It uses infrared fluorescent (IR fluorescent) antibody conjugates to identify strong and weak bands on a western blot. LI-COR adopts a simultaneous ratiometric approach with its 700 and 800 nm infrared (IR) fluorescent detection channels that facilitate a two-color target analysis and normalization.
The platform facilitates protein quantification in a wide linear range and offers flexibility for the blot processing and storage as the signal from the IR fluorescent dyes is robust. LI-COR Odyssey facilitates both chemiluminescent and IR fluorescent protein detection without film usage. This elimination allows flexibility, data archiving, darkroom and developer cost savings, and environmental friendliness.
Key Li-COR benefits include superior sensitivity, quantification, and linear range –
- LI-COR Odyssey near-IR protein quantification discern strong and weak bands on a western blot accurately because fluorescent detection is static, unlike the dynamic light generation in chemiluminescence
- The longer wavelengths of near-IR fluorescent detection result in reduced autofluorescence of membrane surfaces and biomolecules, resulting in lower signal-to-noise ratio and increased sensitivity with NIR fluorophores
- NIR allows a broader, linear detection range such that protein concentrations falling within the detection limits of the instrument are visible in the scanned image
- LI-COR Odyssey compares well in chemiluminescence scenarios, wherein optimization of exposure times for high protein concentrations leads to undetectable/unquantifiable bands for lower protein concentrations and vice-versa