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                                                              • BDNF Biomarker Assay Testing

                                                              Brain-Derived Neurotrophic Factor

                                                              • Brain-Derived Neurotrophic Factor (BDNF) is a member of neurotropin-family of proteins
                                                              • BDNF promotes survival, growth and differentiation of neurons and synapses in central and peripheral nervous system
                                                              • BDNF serves as a biomarker of neurological conditions like schizophrenia and depression
                                                              • BDNF can be assayed using ELISA, MSD or Luminex
                                                              Good Laboratory Practice for Nonclinical Laboratory StudiesFood and Drug Administration (FDA)

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                                                              Click below for answers to your BDNF Biomarker Study questions -
                                                              • What is BDNF?
                                                                What is BDNF?
                                                              • What are BDNF Functions and Effects?
                                                                What are BDNF Functions and Effects?
                                                              • How do we perform BDNF lab test?
                                                                How do we perform BDNF lab test?
                                                              • Where could I learn more about BDNF Assay?
                                                                Where could I learn more about BDNF Assay?

                                                              What is BDNF?

                                                              An Introduction to Brain-Derived Neurotrophic Factor (BDNF)

                                                              Brain-Derived Neurotrophic Factor (BDNF) is a protein encoded by the BDNF gene in humans. It supports the survival of neurons in the central and peripheral nervous systems. BDNF functions as a neurotransmitter modulator and engages in neuronal plasticity. BDNF protein encourages the growth and maturation (differentiation) of new neurons and synapses. BDNF biomarker has been detected in the hippocampus, cortex, and basal forebrain, areas of brain responsible for learning, memory, and higher thinking. BDNF protein is also detectable in the kidneys, retina, motor neurons, saliva, and skeletal muscle . When searching for ‘What is BDNF’, you can also search for its aliases – Abreneurin, ANON2, and BULN2.

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                                                              EGF Functions and Effects

                                                              What are BDNF Functions and Effects?

                                                              Understanding the functions and effects of BDNF

                                                              Brain-Derived Neurotrophic factor is vital for long-term memory. The brain retains the capability of neurogenesis which is the capability to grow neurons from stem cells, even though most human neurons have prenatal formation. BDNF biomarker is one of the neurotropin, which are proteins that aid the stimulation of neurogenesis. Other researched BDNF functions and effects include synaptic plasticity, inflammation and immunity, growth of cardiac and endothelial cells, lipid metabolism, etc. Specific exercises, such as treadmill in mice and aerobics in humans, are considered to increase BDNF growth factors.

                                                              Drug Discovery And Development Process

                                                              Drug Discovery and Development Process

                                                              How long do you think it takes for a new drug to get approved in the US? About 12-15 years, that’s roughly 1/3rd duration of the entire professional career for any of us. Next, how much investment do you think is required to bring a new drug to market?

                                                              Read More at our Learning Center

                                                              How do we perform BDNF lab test?

                                                              Protocols we follow to perform BDNF tests

                                                              BDNF level considered normal in humans and other species is an area of active biological research. At NorthEast BioLab, we tailor BDNF testing to suit your desired assay parameters through BDNF MSD, Luminex, and ELISA. Typically, these BDNF assays employ the fundamentals of the quantitative sandwich enzyme immunoassay technique explained below.
                                                              First, we pre-coat the antibody specific to capture BDNF biomarker onto a microplate and follow up by a plate wash to remove any unbound substances. Next, calibration standards, quality controls, and biological samples get pipetted into the plate wells, and the immobilized capture antibody binds any BDNF present. Then, we wash off any unbound material following proper shaking and incubation as per method protocol. Later, a biotin-conjugated antibody specific for BDNF detection gets added to the wells· After washing, we add avidin conjugated Horseradish Peroxidase (HRP) to the plate wells and rinse again to remove any unbound avidin-enzyme reagent. Eventually, we add a substrate solution to the plate wells, and color develops in proportion to the amount of BDNF bound in the initial step. The final step in BDNF ELISA requires us to halt the color development and quantitate the color intensity with a suitable plate reader.

                                                              Over the years, NorthEast Biolab has acquired the experience to reproduce high-quality BDNF assays. Our scientists and researchers can perform BDNF assay to measure the analyte in multiple formats. We have extensive expertise on BDNF assay specifics, such as matrix type, tissue lysis, volume requirements, sample dilution, and multiplexing possibilities. Our scientists rely only on preapproved vendors and recommended kits, antibodies, diluents, buffers, substrates, reagents, calibrators, control matrices, etc. for running BDNF Luminex and MSD Assay. We follow the industry best practices to ensure accuracy and test your biomarkers through high-affinity materials. Our focus on efficiency and operational excellence helps us deliver high-quality BDNF testing services to all our sponsors.

                                                              EGF lab test

                                                              Registered And Inspected By Various Regulatory Agencies

                                                              learn more about EGF Assay

                                                              Where could I learn more about BDNF Assay?

                                                              Popular BDNF resources for you to explore

                                                              Numerous publications have associated BDNF biomarker levels with conditions affecting various brain functions. Some of these include depression, schizophrenia, obsessive-compulsive disorder, Alzheimer’s disease, Huntington’s disease, Rett syndrome, dementia, and anorexia nervosa and bulimia nervosa neurodegeneration. Increasingly, literature illustrates the considerable interest in the presence of brain-derived neurotrophic factor (BDNF) in human blood.

                                                              Here are some resources for you to read further on the BDNF protein:
                                                              https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504526/
                                                              https://ghr.nlm.nih.gov/gene/BDNF
                                                              https://elifesciences.org/articles/15092/
                                                              https://www.sciencedirect.com/topics/medicine-and-dentistry/brain-derived-neurotrophic-factor/

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