NorthEast BioLab Logo | Advanced Bioanalytical Services Lab Agile Bioanalytical Services CRO
  • Expertise
    • Menu IconAll Expertise
    • Menu IconMass Spectrometry (LC-MS)
      • Menu IconMass Spectrometry Services
      • Menu IconHPLC Testing Services
    • Menu IconMeso Scale Discovery Assay
    • Menu IconELISA Assay
      • Menu IconLuminex Multiplex Assay
    • Menu IconFlow Cytometry
    • Menu IconqPCR Analysis, ddPCR Assay
    • Menu IconWestern Blot, In Cell Western
  • Services
    • Menu IconAll Services
    • Menu IconPharmacokinetics (PK) Study
      • Menu IconToxicology (Tox) Study
      • Menu IconDose Formulation Analysis
      • Menu IconPK PD Analysis
      • Menu IconDrug Metabolism and Pharmacokinetics (DMPK)
      • Menu IconADME Studies
      • Menu IconBioequivalence and Bioavailability Studies
    • Menu IconADA Assay, Neutralizing Antibody Assay
    • Menu IconMultiplex Cytokine Assay
    • Menu IconBiomarker Assay
      • Menu IconBiomarker Assay List
    • Menu IconCell-Based Assay
      • Menu IconMechanism of Action (MOA) Studies
      • Menu IconCytotoxicity Assay
      • Menu IconBiochemical Assay
    • Menu IconBioanalytical Assay Validation
  • Solutions
    • Menu IconAll Solutions
    • Menu IconClinical CRO
      • Menu IconCentral Lab Services
      • Menu IconPBMC Services
    • Menu IconGLP Laboratory
      • Menu IconBioanalysis
      • Menu IconNon-GLP Testing
    • Menu IconPreclinical CRO
    • Menu IconMethod Development, Validation, And Transfer
      • Menu IconMethod Validation
      • Menu IconMethod Development
      • Menu IconMethod Transfer
    • Menu IconAnalytical Testing
      • Menu IconDiscovery Screening Services
      • Menu IconAnalytical Quantitation
      • Menu IconCosmetic Testing
  • About Us
    • Menu IconAbout Us
    • Menu IconOverview
    • Menu IconLeadership
    • Menu IconFacility and Equipment
    • Menu IconQuality and Compliance
    • Menu IconTherapeutic Areas
    • Menu IconWhy Us?
    • Menu IconValues
    • Menu IconProcess
    • Menu IconPartners
    • Menu IconCareers
      • Business Development Associate
      • Sr. Scientist – Cell Based Assay
      • GLP Quality Assurance Manager
      • Sr. Scientist, ELISA Immunoassay Bioanalysis
      • Sr. Scientist, LC-MS/MS Bioanalysis
      • Associate Scientist, Central Laboratory Services
      • Sr. Scientist - Pharmacokinetics (PK)
      • Content Manager, Scientific and Regulatory Writing
  • Resources
    • Menu IconLearning Center
    • Menu IconBlog
    • Menu IconCase Study
    • Menu IconVideo Brochures
    • Menu IconNews and Events
    • Menu IconTestimonials
    • Menu IconContact Us
  • Request Quote
NorthEast BioLab Logo | Advanced Bioanalytical Services Lab Agile Bioanalytical Services CRO Request Quote

Expertise

  • Menu IconAll Expertise
    • Menu IconMass Spectrometry (LC-MS)
      • Menu IconMass Spectrometry Services
      • Menu IconHPLC Testing Services
    • Menu IconMeso Scale Discovery Assay
      • Menu IconELISA Assay
        • Menu IconLuminex Multiplex Assay
      • Menu IconFlow Cytometry
        • Menu IconqPCR Analysis, ddPCR Assay
          • Menu IconWestern Blot, In Cell Western

            Services

            • Menu IconAll Services
              • Menu IconPharmacokinetics (PK) Study
                • Menu IconToxicology (Tox) Study
                • Menu IconDose Formulation Analysis
                • Menu IconPK PD Analysis
                • Menu IconDrug Metabolism and Pharmacokinetics (DMPK)
                • Menu IconADME Studies
                • Menu IconBioequivalence and Bioavailability Studies
              • Menu IconADA Assay, Neutralizing Antibody Assay
                • Menu IconMultiplex Cytokine Assay
                  • Menu IconBiomarker Assay
                    • Menu IconBiomarker Assay List
                  • Menu IconCell-Based Assay
                    • Menu IconMechanism of Action (MOA) Studies
                    • Menu IconCytotoxicity Assay
                    • Menu IconBiochemical Assay
                  • Menu IconBioanalytical Assay Validation

                    Solutions

                    • Menu IconAll Solutions
                      • Menu IconClinical CRO
                        • Menu IconCentral Lab Services
                        • Menu IconPBMC Services
                      • Menu IconGLP Laboratory
                        • Menu IconBioanalysis
                        • Menu IconNon-GLP Testing
                      • Menu IconPreclinical CRO
                        • Menu IconMethod Development, Validation, And Transfer
                          • Menu IconMethod Validation
                          • Menu IconMethod Development
                          • Menu IconMethod Transfer
                        • Menu IconAnalytical Testing
                          • Menu IconDiscovery Screening Services
                          • Menu IconAnalytical Quantitation
                          • Menu IconCosmetic Testing

                        About Us

                        • Menu IconAbout Us
                          • Menu IconOverview
                            • Menu IconLeadership
                              • Menu IconFacility and Equipment
                                • Menu IconQuality and Compliance
                                  • Menu IconTherapeutic Areas
                                    • Menu IconWhy Us?
                                      • Menu IconValues
                                        • Menu IconProcess
                                          • Menu IconPartners
                                            • Menu IconCareers
                                              • Menu IconBusiness Development Associate
                                              • Menu IconSr. Scientist – Cell Based Assay
                                              • Menu IconGLP Quality Assurance Manager
                                              • Menu IconSr. Scientist, ELISA Immunoassay Bioanalysis
                                              • Menu IconSr. Scientist, LC-MS/MS Bioanalysis
                                              • Menu IconAssociate Scientist, Central Laboratory Services
                                              • Menu IconSr. Scientist - Pharmacokinetics (PK)
                                              • Menu IconContent Manager, Scientific and Regulatory Writing

                                            Resources

                                            • Menu IconLearning Center
                                              • Menu IconBlog
                                                • Menu IconCase Study
                                                  • Menu IconVideo Brochures
                                                    • Menu IconNews and Events
                                                      • Menu IconTestimonials
                                                        • Menu IconContact Us

                                                          Request Quote

                                                            Contact Us

                                                            NorthEast BioLab Logo Agile Bioanalysis Services CRO
                                                            Request Quote Contact Us
                                                            • Home
                                                            • IFN ɣ Biomarker Assay Testing

                                                            Interferon-gamma

                                                            • Interferon-gamma/ IFN γ is a cytokine with role in crucial role in innate and adaptive immunity
                                                            • IFN γ stimulates macrophage and induces Class II MHC expression.
                                                            • IFN γ is a biomarker of autoimmune and auto-inflammatory diseases
                                                            • IFN γ 2 can be assayed using immune assays ELISA, MSD or Luminex

                                                            Regulated And Inspected By:

                                                            Good Laboratory Practice for Nonclinical Laboratory Studies | NorthEast BioLab Food and Drug Administration (FDA | NorthEast BioLab Clinical Laboratory Improvement Amendments | NorthEast BioLab International Orgaization for Standardization |NorthEast BioLab

                                                            Provide Your Bioanalysis Study Details

                                                            We Will Respond Within 1 Business Day

                                                            Click below for answers to your IFN γ Biomarker Study questions -
                                                            • What is IFN ɣ ?
                                                              What is IFN ɣ ?
                                                            • What are IFN ɣ Functions and Effects?
                                                              What are IFN ɣ Functions and Effects?
                                                            • How do we perform IFN ɣ lab test?
                                                              How do we perform IFN ɣ lab test?
                                                            • Where could I learn more about IFN ɣ Assay?
                                                              Where could I learn more about IFN ɣ Assay?

                                                            What is IFN ɣ?

                                                            An Introduction to Interferon-gamma (IFN ɣ)

                                                            Interferon-gamma or IFN γ or is a human dimerized soluble protein encoded by the IFNG gene. The biologically active IFN γ is an interlocking anti-parallel form of two monomers. IFN γ is the sole member of the type II IFN group. IFN γ binds to the IFN-γ receptor complex to elicit a cellular signal. Two subunits, IFNGR1 and IFNGR2, make up the IFN-γ receptor complex. IFN γ plays a crucial role in innate and adaptive immunity. It inhibits viral replication, fights against bacteria, and modulates and stimulates the immune system.
                                                            When you look up “What is Interferon Gamma?” you may come across its former name, immune interferon.

                                                            Sponsors Love Our Bioanalytical Laboratory Services For Top Quality And Industry Leading Turnaround

                                                            Connect With Us For A Reference Call With Them!

                                                            User Vector
                                                            VP, Bioanalytical Development

                                                            “NorthEast BioLab is the most responsive and thorough bioanalysis lab services CRO.”

                                                            User Vector
                                                            VP, Biomarker Development 

                                                            NorthEast BioLab is a responsive, collaborative, and reliable partner

                                                            User Vector
                                                            Executive Director, Pharmacokinetics

                                                            We trust NorthEast BioLab to design and execute streamlined, impactful bioanalytical projects

                                                            User Vector
                                                            President & CSO, Biotech

                                                            We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data

                                                            User Vector
                                                            Sr. Associate Dir., Clinical Trials

                                                            We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

                                                            User Vector
                                                            Co-Founder, Biotech & University PI

                                                            NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism

                                                            User Vector
                                                            VP, PK PD Analysis

                                                            NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

                                                            User Vector
                                                            Head, Bioanalytical Development

                                                            “NorthEast BioLab goes the extra mile. We look forward to more meaningful collaborations.”

                                                            User Vector
                                                            Sr. Dir., Bioanalytical Development & QC

                                                            NorthEast BioLab offers a science-based, hands-on approach to the latest bioanalytical platforms

                                                            User Vector
                                                            SVP Quality & Compliance, Biotech

                                                            We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

                                                            User Vector
                                                            Director, PK

                                                            We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

                                                            User Vector
                                                            VP, Head of Clinical Operations

                                                            Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

                                                            User Vector
                                                            Executive Director, Drug Research

                                                            NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

                                                            User Vector
                                                            Dir., Bioequivalence Testing

                                                            Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

                                                            User Vector
                                                            Head, PK

                                                            This study, same as all other bioanalytical studies, was completed with top quality and reporting standard with incredible responsiveness.

                                                            User Vector
                                                            VP, Development Operations

                                                            NorthEast BioLab’s scientists deliver high-quality data on time and within budget

                                                            Tackle Your IFN ɣ Biomarker Assay Testing With Our Veteran Team Now!

                                                            SPEAK TO OUR SCIENTISTS

                                                            How do we perform IFN ɣ lab test?

                                                            Protocols we follow to perform IFN γ tests

                                                            We, at NorthEast BioLabs, perform IFN γ assays including the interferon gamma release assay, mouse interferon gamma ELISA, gamma interferon test, and interferon gamma assay test to suit your desired testing parameters. These assays and tests are done via Luminex, MSD, and ELISA. In this, in vitro quantitative colorimetric detection assays, we detect human plasma or serum, cell culture, cell culture supernatant, cell lysates, and tissue lysates. Following is an ELISA protocol that uses the interferon gamma protein in the Sandwich-Based Technique.

                                                            We begin with a 100 μl sample or standard in each well precoated with antibodies against IFN γ. We then incubate it for 2.5 hrs at room temperature (RT) or overnight at 4 ℃. Biotin antibody is introduced in each well and incubated for 1 hr at RT. We then add 100 μl of Streptavidin-peroxidase solution to each well and incubate it for 45 mins at RT. This is followed by 100 μl TMB One-Step Substrate reagent for coloring and again incubated for 30 mins at RT. We then add 50 μl of Stop Solution to each well at the end of the reaction. The samples are read at 450 nm immediately.

                                                            This Quantitative Sandwich assay has a detection range of 15000 pg/ml to 15 pg/ml with a sensitivity of 15 pg/ml.

                                                            EGF lab test

                                                            Registered And Inspected By Various Regulatory Agencies

                                                            • Good Laboratory Practice for Nonclinical Laboratory Studies | NorthEast BioLab
                                                            • Food and Drug Administration (FDA | NorthEast BioLab
                                                            • clia
                                                            • United States Drug Enforcement Administration | NorthEast BioLab
                                                            • Department of Consumer Protection | NorthEast BioLab

                                                            Don’t Wait, Start Your IFN ɣ Biomarker Assay Testing Today!

                                                            SPEAK TO OUR SCIENTISTS

                                                            Where could I learn more about IFN ɣ Assay?

                                                            Popular IFN γ resources for you to explore

                                                            IFN γ has received mixed therapeutic responses. Cells express recombinant human IFN γ in different systems including protozoan, prokaryotic, fungal, insect, and mammalian cells. ACTIMMUNE, a marketed human IFN γ is not glycosylated and has a short half-life post-injection in the bloodstream. The purification system from the bacteria is also expensive, making it a costly biopharmaceutical.

                                                            IFN γ is still not an approved drug in cancer immunotherapy. IFN γ studies including interferon gamma assay test, interferon gamma ELISA, mouse interferon gamma ELISA, and interferon gamma release assay have observed improved survival in patients with melanoma and carcinoma cancers. IFN γ has shown promising results in stage 2 and 3 ovarian carcinoma patients. Studies have observed that CD8-positive secretes IFN γ that up-regulates PD-L1 in ovarian cancer cells and enhances tumor growth. Invitro IFN γ cancer studies report anti-proliferative activity that causes cell death or inhibits cellular growth.

                                                            Exciting research is undertaken on IFN γ. Explore more about IFN γ with the following references.
                                                            https://www.frontiersin.org/articles/10.3389/fimmu.2018.00847/full
                                                            https://www.sciencedirect.com/topics/neuroscience/interferon-gamma
                                                            https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154595/
                                                            https://www.pnas.org/content/114/33/E6867
                                                            https://www.liebertpub.com/doi/full/10.1089/jir.2018.0087

                                                            Multiplex Cytokine Analysis, MSD Cytokine Assay

                                                            Understand Multiplex Cytokine Analysis, Meso Scale Discovery (MSD) Cytokine Assay For Inflammation, Chemokines, Interleukins, Growth Factor …

                                                            Read More
                                                            Cytokine Video
                                                            Biomarker Assay List | NorthEast BioLab
                                                            Analytical Laboratory Services | NorthEast BioLab

                                                            What are IFN ɣ Functions and Effects?

                                                            Understanding the functions and effects of IFN γ

                                                            Once cells express IFN γ, it activates the macrophage and induces Class II major histocompatibility complex (MHC) expression. Studies have associated abnormal interferon gamma structure or IFN γ expression with several autoimmune and auto-inflammatory diseases. Because of its antiviral, immunomodulatory, and immunostimulatory effects, the interferon gamma inducing factor of IFN γ plays a vital role in immune system responses. As part of innate immune response, as studied in interferon gamma release assays, natural killer cells and natural killer T cells produce IFN γ, and as part of the adaptive immune response, CD4 Th1 and CD8 cytotoxic T-lymphocytes produce IFN γ.

                                                            Research with mouse interferon gamma ELISA has shown that IFN γ facilitates transcription of 30 genes involved in physiological and cellular responses.
                                                            Some fundamental effects of anti-interferon gamma antibodies are:

                                                            • Promotes Natural Killer cell activity
                                                            • Enhances macrophage lysosomal activity and antigen presentation
                                                            • Production of IgG2a and IgG3
                                                            • Increased expression of Class I MHC molecules on normal cells
                                                            • Increased expression of Class II MHC molecules on antigen-presenting cells
                                                            • Promotes binding of intrinsic defense factors

                                                            Trust Our IFN ɣ Biomarker Assay Testing.

                                                            SPEAK TO OUR SCIENTISTS

                                                            Explore More Biomarkers

                                                            • EGF lab test Brain-Derived Neurotrophic Factor
                                                            • Granulate Colony Stimulation Factor
                                                            • Preclinical Research Services | NorthEast BioLab Epidermal growth factor
                                                            • Clinical Study | NorthEast BioLab Fas Ligand
                                                            • Granulocyte Macrophage Colony Stimulating Factor
                                                            • Leukemia Inhibitory Factor
                                                            • Luteinizing hormone
                                                            • Macrophage Migration Inhibitory Factor
                                                            • Interferon Beta
                                                            • Interferon Gamma
                                                            • Transforming Growth Factor Beta 1
                                                            • B Type Natriuretic Peptide
                                                            • DMPK Services | NorthEast BioLab Glial Fibrillary Acidic Protein
                                                            • Preclinical Study | NorthEast BioLab Cystatin C
                                                            • Hepatocyte Growth Factor
                                                            • Interferon Alpha 2
                                                            • Tumor Necrosis Factor Alpha

                                                            Contact A Scientist With Your Bioanalytical Study Details

                                                            We Will Respond Within 1 Business Day

                                                            Expertise

                                                            Expertise

                                                            • Mass Spectrometry (LC-MS)
                                                            • Meso Scale Discovery Assay
                                                            • ELISA Assay
                                                            • Flow Cytometry
                                                            • qPCR Analysis, ddPCR Assay
                                                            • Western Blot, In Cell Western

                                                            Services

                                                            Services

                                                            • Pharmacokinetics (PK) Study
                                                            • ADA Assay, Neutralizing Antibody Assay
                                                            • Multiplex Cytokine Assay
                                                            • Biomarker Assay
                                                            • Cell-Based Assay
                                                            • Bioanalytical Assay Validation

                                                            Solutions

                                                            Solutions

                                                            • Clinical CRO
                                                            • GLP Laboratory
                                                            • Preclinical CRO
                                                            • Method Development, Validation, And Transfer
                                                            • Analytical Testing

                                                            About Us

                                                            About Us

                                                            • Overview
                                                            • Leadership
                                                            • Facility and Equipment
                                                            • Quality and Compliance
                                                            • Therapeutic Areas
                                                            • Why Us?
                                                            • Values
                                                            • Process
                                                            • Partners
                                                            • Careers

                                                            Resources

                                                            Resources

                                                            • Learning Center
                                                            • Blog
                                                            • Case Study
                                                            • Video Brochures
                                                            • News and Events
                                                            • Testimonials
                                                            • Contact Us
                                                            NorthEast BioLab Logo Agile Bioanalytical Services CRO
                                                            sales@nebiolab.comFollow Up On Email
                                                            (203) 361 3780Follow Up On Phone

                                                            35 Worth Ave, Hamden, CT 06518

                                                            • Good Laboratory Practice for Nonclinical Laboratory Studies | NorthEast BioLab
                                                            • Food and Drug Administration (FDA | NorthEast BioLab
                                                            • clia
                                                            • United States Drug Enforcement Administration | NorthEast BioLab
                                                            • Department of Consumer Protection | NorthEast BioLab
                                                            • Privacy Policy
                                                            • Terms of Use
                                                            • Sitemap
                                                            All rights reserved.