How do we perform IFN ɣ lab test?
Protocols we follow to perform IFN γ tests
We, at NorthEast BioLabs, perform IFN γ assays including the interferon gamma release assay, mouse interferon gamma ELISA, gamma interferon test, and interferon gamma assay test to suit your desired testing parameters. These assays and tests are done via Luminex, MSD, and ELISA. In this, in vitro quantitative colorimetric detection assays, we detect human plasma or serum, cell culture, cell culture supernatant, cell lysates, and tissue lysates. Following is an ELISA protocol that uses the interferon gamma protein in the Sandwich-Based Technique.
We begin with a 100 μl sample or standard in each well precoated with antibodies against IFN γ. We then incubate it for 2.5 hrs at room temperature (RT) or overnight at 4 ℃. Biotin antibody is introduced in each well and incubated for 1 hr at RT. We then add 100 μl of Streptavidin-peroxidase solution to each well and incubate it for 45 mins at RT. This is followed by 100 μl TMB One-Step Substrate reagent for coloring and again incubated for 30 mins at RT. We then add 50 μl of Stop Solution to each well at the end of the reaction. The samples are read at 450 nm immediately.
This Quantitative Sandwich assay has a detection range of 15000 pg/ml to 15 pg/ml with a sensitivity of 15 pg/ml.