How do we perform IFNα2 lab test?
Protocols we follow to perform IFNα2 tests
At NorthEast BioLabs, we perform Luminex, ELISA, and MSD assays to detect alpha interferons and beta interferons in plasma, serum, cell culture supernatants, tissue homogenates, cell lysates, and other fluids. Following, we have outlined a double-antibody Sandwich ELISA assay protocol.
We begin the IFN Alpha testing with a pre-coated microtiter plate with an IFNα 2 specific antibody. We add 100 μl of sample or standard to the wells and introduce a biotin-conjugated antibody in each well. This antibody is specific to IFNα 2. Next, we incubate Avidin conjugated Horseradish Peroxidase in each well. Post-incubation, we add a TMB reporter molecule. Wells containing all three, IFNα 2, enzyme-conjugated Avidin, and Biotin-conjugated antibodies will show a color change. To end the reaction we add sulphuric acid and measure the color change spectrophotometrically at 450nm (± 10nm). We then correlate optical density (O.D) of the sample, with the standard curve and determine the concentration of IFNα 2 in the samples.
The anti-interferon assay employs the principle of immunosorbent assay and colorimetric system to identify IFNα 2 in the samples. The assay has a detection range between 15.6 pg/ml – 1000 pg/ml, with minimum detectable IFNα 2 doses of typically < 6.2 pg/ml. The assay is highly precise, with an intra-assay precision of < 10% and inter-assay precision of <12%.