sales@nebiolab.com Email Your Inquiry
(203) 361 3780 Speak to Our Scientists
Agile Bioanalysis Services CRO
  • Expertise
    • All Expertise
    • Liquid Chromatography Mass Spectrometry
      • HPLC Method Development Validation
      • Mass Spectrometric Analysis
    • ELISA Method
    • Meso Scale Discovery Assay
    • Luminex Multiplex Assay
    • LI-COR Odyssey Assay
    • PK PD Analysis
    • Method Development, Validation, and Transfer
      • Method Validation
      • Method Development
      • Method Transfer
  • Services
    • All Services
    • Pharmacokinetics (PK) Study
      • Drug Metabolism and Pharmacokinetics (DMPK)
      • ADME Studies
    • Toxicology (Tox) Study
      • Dose Formulation Analysis
    • Immunogenicity Testing
    • Biomarker Testing Services
      • Biomarker Assay List
    • Bioequivalence and Bioavailability Studies
    • Bioanalytical Assay
      • Biochemical Assay
      • Mechanism of Action (MOA) Studies
      • Cell-based Assays
      • Cytotoxicity Assay
  • Solutions
    • All Solutions
    • Bioanalysis
    • GLP Laboratory
    • Clinical CRO
      • Clinical Trial Sample Management and Storage
    • Preclinical CRO
    • Analytical Testing
      • Analytical Quantitation
    • Non-GLP Testing
      • Discovery and Research
      • Cosmetic Testing
  • About Us
    • About Us
    • Overview
    • Why Us?
    • Values
    • Leadership
    • Process
    • Quality and Compliance
    • Facility and Equipment
    • Therapeutic Areas
    • Partners
    • Careers
      • GLP Quality Assurance Manager, Bioanalytical Lab
      • Sr. Scientist, ELISA Immunoassay Bioanalysis
      • Sr. Scientist, LC-MS/MS Bioanalysis – Hamden, CT, USA
      • Associate Scientist, Central Laboratory Services
      • Sr. Scientist – Pharmacokinetics (PK) Studies
      • Content Manager, Scientific and Regulatory Writing
  • Resources
    • Learning Center
    • Blog
    • Case Study
    • News and Events
    • Testimonials
    • Contact Us
  • Request Quote
Agile Bioanalysis Services CRO Request Quote

Expertise

  • All Expertise
    • Liquid Chromatography Mass Spectrometry
      • HPLC Method Development Validation
      • Mass Spectrometric Analysis
    • ELISA Method
      • Meso Scale Discovery Assay
        • Luminex Multiplex Assay
          • LI-COR Odyssey Assay
            • PK PD Analysis
              • Method Development, Validation, and Transfer
                • Method Validation
                • Method Development
                • Method Transfer

              Services

              • All Services
                • Pharmacokinetics (PK) Study
                  • Drug Metabolism and Pharmacokinetics (DMPK)
                  • ADME Studies
                • Toxicology (Tox) Study
                  • Dose Formulation Analysis
                • Immunogenicity Testing
                  • Biomarker Testing Services
                    • Biomarker Assay List
                  • Bioequivalence and Bioavailability Studies
                    • Bioanalytical Assay
                      • Biochemical Assay
                      • Mechanism of Action (MOA) Studies
                      • Cell-based Assays
                      • Cytotoxicity Assay

                    Solutions

                    • All Solutions
                      • Bioanalysis
                        • GLP Laboratory
                          • Clinical CRO
                            • Clinical Trial Sample Management and Storage
                          • Preclinical CRO
                            • Analytical Testing
                              • Analytical Quantitation
                            • Non-GLP Testing
                              • Discovery and Research
                              • Cosmetic Testing

                            About Us

                            • About Us
                              • Overview
                                • Why Us?
                                  • Values
                                    • Leadership
                                      • Process
                                        • Quality and Compliance
                                          • Facility and Equipment
                                            • Therapeutic Areas
                                              • Partners
                                                • Careers
                                                  • GLP Quality Assurance Manager, Bioanalytical Lab
                                                  • Sr. Scientist, ELISA Immunoassay Bioanalysis
                                                  • Sr. Scientist, LC-MS/MS Bioanalysis – Hamden, CT, USA
                                                  • Associate Scientist, Central Laboratory Services
                                                  • Sr. Scientist - Pharmacokinetics (PK) Studies
                                                  • Content Manager, Scientific and Regulatory Writing

                                                Resources

                                                • Learning Center
                                                  • Blog
                                                    • Case Study
                                                      • News and Events
                                                        • Testimonials
                                                          • Contact Us

                                                            Request Quote

                                                              Contact Us

                                                              NorthEast BioLab Logo Agile Bioanalysis Services CRO
                                                              Request Quote Contact Us
                                                              • Home
                                                              • GFAP Biomarker Assay Testing

                                                              Glial fibrillary acidic protein

                                                              • Glial fibrillary acidic protein/GFAP is a type III intermediate filament protein
                                                              • GFAP function is not fully understood but is highly expressed in CNS tissues
                                                              • GFAP may serve as a biomarker of CNS disorders including Alexander disease
                                                              • GFAP can be assayed using immunoreactive based assays: ELISA, MSD or Luminex
                                                              Good Laboratory Practice for Nonclinical Laboratory StudiesFood and Drug Administration (FDA)

                                                              Provide Your Bioanalysis Study Details

                                                              We Will Contact You Within 1 Business Day

                                                              Click below for answers to your GFAP Biomarker Study questions -
                                                              • What is GFAP?
                                                                What is GFAP?
                                                              • What are GFAP Functions and Effects?
                                                                What are GFAP Functions and Effects?
                                                              • How do we perform GFAP lab test?
                                                                How do we perform GFAP lab test?
                                                              • Where could I learn more about GFAP Assay?
                                                                Where could I learn more about GFAP Assay?

                                                              What is GFAP?

                                                              An Introduction to Glial fibrillary acidic protein (GFAP)

                                                              Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein belonging to the intermediate filament protein family. The GFAP gene in humans encodes the GFAP protein. The gene is located on chromosome 17. Several CNS cell types that include ependymal cells and astrocytes express GFAP proteins during development. Other human cells, such as keratinocytes, Leydig cells, chondrocytes, and osteocytes, also express GFAP. GFAP, along with the other three non-epithelial cells belonging to the same protein family, regulates the functions and structure of the cytoskeleton. Although many studies use GFAP as a cell marker, we have still not completely understood its role in the body.

                                                              Studies suggest a GFAP protein can help maintain cell shape and astrocyte mechanical strength. Type III intermediate filament proteins comprise three domains, head, rod, and tail. The rod domain may differ in its DNA sequence, but its protein structure is highly conserved. GFAP can form both a homodimer and heterodimer and can polymerize other proteins in the type III group. GFAP comprises eight different isoforms that make up distinct astrocyte subpopulations in the human brain. Out of these isoforms, GFAP alpha is the only one that can assemble homomerically. Another isoform, GFAP delta, is one of the most researched GFAP isoforms. Studies have linked GFAP delta isoform with neural stem cells.

                                                              You can also use the other names like GFAP and ALXDRD when searching for ‘What is GFAP?’ or ‘What is Glial fibrillary acidic protein’?

                                                              What Our Sponsors Have to Say?

                                                              VP, Bioanalytical Development

                                                              NorthEast BioLab is by far the most responsive and thorough bioanalysis lab services CRO.

                                                              VP, Biomarker Development 

                                                              We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.

                                                              Executive Director, Pharmacokinetics

                                                              We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects.

                                                              President & CSO, Biotech

                                                              We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data.

                                                              Sr. Associate Director, Clinical Trials

                                                              We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

                                                              Co-Founder, Biotech & University PI

                                                              NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism.

                                                              VP, PK PD Analysis

                                                              NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

                                                              Head, Bioanalytical Development

                                                              NorthEast BioLab truly goes that extra mile, and we hope to continue enjoying seminal interactions with them.

                                                              Sr.Director, Bioanalytical Development & QC

                                                              NorthEast BioLab presents a science-based, hands-on, no-frills approach on the latest bioanalytical platforms.

                                                              SVP Quality & Compliance, Biotech

                                                              We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

                                                              Director, PK

                                                              We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

                                                              VP, Head of Clinical Operations

                                                              Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

                                                              Executive Director, Drug Research

                                                              NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

                                                              Director, Bioequivalence Testing

                                                              Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

                                                              Head, PK

                                                              This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with top quality and reporting standard with incredible responsiveness.

                                                              VP, Development Operations

                                                              As a small company, our vendors are key members of our project teams. The scientists at NorthEast BioLab are technical experts, who produce high-quality data, on-time, and on-budget.

                                                              Ready to Take the First Step?

                                                              Consult with a Scientist About Your GFAP Biomarker Assay Study
                                                              SPEAK TO OUR SCIENTISTS
                                                              EGF Functions and Effects

                                                              What are GFAP Functions and Effects?

                                                              Understanding the functions and effects of GFAP

                                                              As astrocytes express GFAP, it regulates crucial CNS processes that include cellular communication and efficient functioning of the blood-brain barrier. Studies have shown that the GFAP gene adjusts its filament network and regulates mitosis in the cell. Studies of the GFAP biomarker in knockout mice induce white matter structure deterioration, abnormal myelination, and deterioration of the blood-brain barrier. On the other hand, in GFAP knockout mice, Purkinje cells were unable to show their original structure, and these mice perform inefficiently in conditioning experiments. In vitro studies that use antisense RNA show that astrocytes lacking GFAP protein are incapable of forming normal neuronal extensions. GFAP hence, plays a critical role in CNS injury repair, especially to form glial scars throughout the CNS.

                                                              Drug Discovery And Development Process

                                                              Drug Discovery and Development Process

                                                              How long do you think it takes for a new drug to get approved in the US? About 12-15 years, that’s roughly 1/3rd duration of the entire professional career for any of us. Next, how much investment do you think is required to bring a new drug to market?

                                                              Read More at our Learning Center

                                                              How do we perform GFAP lab test?

                                                              Protocols we follow to perform GFAP tests

                                                              We perform MSD, Luminex, and ELISA assays for detecting GFAP biomarker in plasma, serum, and other biological fluids. Following is a Sandwich-based ELISA protocol used to detect GFAP protein in any biological fluids.

                                                              We begin the GFAP test with a microtiter plate, pre-coated with a GFAP-specific antibody. We then add 100 μl of standard or samples, followed by a GFAP specific biotin-conjugated antibody. Next, we incubate the wells with Avidin-conjugated HRP. We then add a TMB substrate solution in each well. The wells containing all three biotin-conjugated antibodies, GFAP, and enzyme-conjugated Avidin exhibit a change in color. Lastly, we end the reaction by adding a sulphuric acid solution and measure the color change spectrophotometrically at 450 nm (± 10 nm). We then compare the Optical Density (OD) with the standard curve and determine GFAP concentration.

                                                              The assay for GFAP immunoreactivity has a detection range of 31.2 pg/ml to 2000 pg/ml with a minimum detectable dose of < 10.2 pg/ml. The GFAP assay is highly specific, with an intra-assay precision of < 10% and inter-assay precision of <12%.

                                                              EGF lab test

                                                              Registered And Inspected By Various Regulatory Agencies

                                                              learn more about EGF Assay

                                                              Where could I learn more about GFAP Assay?

                                                              Popular GFAP resources for you to explore

                                                              Studies have associated improper GFAP regulation with several disorders. GFAP dysregulation induces glial cells to cause detrimental injuries. Post-injury, the cells upregulate GFAP protein levels, and they interact with astrocytes and fibrous tissues to form a scar that re-establishes the glial margins. Alexander disease is another condition directly related to the presence of the GFAP. Alexander disease is a rare genetic disorder with symptoms including dementia, mental and physical retardation, seizures, and head and brain size enlargements.

                                                              To learn more about the biological role of GFAP and GFAP immunoreactivity, explore the resources below.
                                                              https://ghr.nlm.nih.gov/gene/GFAP
                                                              https://www.genecards.org/cgi-bin/carddisp.pl?gene=GFAP
                                                              https://www.uniprot.org/uniprot/P14136
                                                              https://www.omim.org/entry/137780
                                                              https://www.sciencedirect.com/topics/neuroscience/glial-fibrillary-acidic-protein

                                                              Our Experienced Scientists Are Ready To Help You, Today!
                                                              SPEAK TO OUR SCIENTISTS

                                                              Explore More Biomarkers

                                                              • Brain-Derived Neurotrophic Factor Brain-Derived Neurotrophic Factor
                                                              • Granulate Colony Stimulation Factor Granulate Colony Stimulation Factor
                                                              • Epidermal growth factor Epidermal growth factor
                                                              • Fas Ligand Fas Ligand
                                                              • Granulocyte Macrophage Colony Stimulating Factor Granulocyte Macrophage Colony Stimulating Factor
                                                              • Leukemia Inhibitory Factor Leukemia Inhibitory Factor
                                                              • Luteinizing hormone Luteinizing hormone
                                                              • Macrophage Migration Inhibitory Factor Macrophage Migration Inhibitory Factor
                                                              • Interferon Beta Interferon Beta
                                                              • Interferon Gamma Interferon Gamma
                                                              • Transforming Growth Factor Beta 1 Transforming Growth Factor Beta 1
                                                              • B Type Natriuretic Peptide B Type Natriuretic Peptide
                                                              • Glial Fibrillary Acidic Protein Glial Fibrillary Acidic Protein
                                                              • Cystatin C Cystatin C
                                                              • Hepatocyte Growth Factor Hepatocyte Growth Factor
                                                              • Interferon Alpha 2 Interferon Alpha 2
                                                              • Tumor Necrosis Factor Alpha Tumor Necrosis Factor Alpha

                                                              Expertise

                                                              • Liquid Chromatography Mass Spectrometry
                                                              • ELISA Method
                                                              • Meso Scale Discovery Assay
                                                              • Luminex Multiplex Assay
                                                              • LI-COR Odyssey Assay
                                                              • PK PD Analysis
                                                              • Method Development Validation and Transfer

                                                              Services

                                                              • Pharmacokinetics (PK) Study
                                                              • Toxicology (Tox) Study
                                                              • Immunogenicity Testing
                                                              • Biomarker Testing Services
                                                              • Bioequivalence and Bioavailability Studies
                                                              • Bioanalytical Assay

                                                              Solutions

                                                              • Bioanalysis
                                                              • GLP Laboratory
                                                              • Clinical CRO
                                                              • Preclinical CRO
                                                              • Analytical Testing
                                                              • Non-GLP Testing

                                                              About Us

                                                              • Overview
                                                              • Why Us?
                                                              • Values
                                                              • Leadership
                                                              • Process
                                                              • Quality and Compliance
                                                              • Facility and Equipment
                                                              • Therapeutic Areas
                                                              • Partners
                                                              • Careers

                                                              Resources

                                                              • Learning Center
                                                              • Blog
                                                              • Case Study
                                                              • News and Events
                                                              • Testimonials
                                                              • Contact Us
                                                              NorthEast BioLab Logo

                                                              35 Worth Ave, Hamden, CT 06518

                                                              sales@nebiolab.com
                                                              (203) 361 3780

                                                              Consult with a Scientist Now

                                                              We Will Contact You Within 1 Business Day

                                                              • Good Laboratory Practice for Nonclinical Laboratory Studies
                                                              • Food and Drug Administration (FDA)
                                                              • United States Drug Enforcement Administration
                                                              • Connecticut State Department of Consumer Protection
                                                              • Privacy Policy
                                                              • Terms of Use
                                                              • Sitemap
                                                              © 2019 - 2025, NorthEast BioAnalytical Laboratories LLC. All rights reserved.

                                                              How Can We Help?

                                                              I agree to receive emails, calls, and messages, etc from NorthEast BioLab.

                                                              Before you go! Get
                                                              your FREE Download:

                                                              Top 7 Hidden Pitfalls to your Bioanalytical Milestones

                                                              Step by step guide on how to accelerate your drug approval

                                                              • Addressing Main Constraints
                                                              • Research Best Practices
                                                              • The Economic Advantage
                                                              • Critical Decision Marketing Mode Easy
                                                              • Legal Documentation Checklist
                                                              We won’t share your email, ever, See our privacy policy

                                                              No thanks, I don’t need to improve my drug approval process