Target identification and validation
- Metabolic activity and functional behavior of biomolecules
- Protein-protein, protein-RNA, and protein-DNA interactions
- Small interfering RNA (siRNA) and gain-of-function studies
Assay development
- Biochemical assays with expression and purification of proteins
- Molecular Biology, including cloning, mutant generation, etc.
- Stable mammalian cell line generation
Lead optimization
- “Mechanism of action”, including pathway identification
- Compound profiling and pharmacology – early efficacy, safety, and toxicity
