Mass Spec Analysis measures the mass-to-charge ratio of analyte ions in a sample of interest to identify and quantify molecules, determine their structure and composition, and study their interactions and functions. This technique has applications in various scientific fields such as proteomics, metabolomics, pharmaceuticals, environmental, and forensic sciences.
Mass Spectrometry Analysis services include performing different bioanalytical assay applications using state-of-the-art equipment and expertise. Generally, a Mass Spectrometry service company offers this bioanalytical solution to researchers, industries, and agencies who need to analyze their samples using Mass Spectrometry. Mass Spectrometry analysis allows customers to find answers to their scientific questions, solve analytical problems, and achieve their research or business goals. NorthEast BioLab is an outstanding mass spectrometry company offering Mass Spec services for over two decades.
Over the last decade, Mass Spectrometry Analysis has become integral to drug development and research. It accelerates each phase of drug R&D, providing invaluable insight for driving drug innovation in the right direction. From target identification to regulatory approval, Mass Spectrometry Analysis plays a crucial role in identifying drug targets, characterizing drug candidates, optimizing drug properties, evaluating safety and efficacy, and monitoring drug metabolism and pharmacokinetics. Mass Spectrometry testing services also support quality control, bioequivalence studies, and post-marketing surveillance of drugs.
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NorthEast BioLab is by far the most responsive and thorough bioanalysis lab services CRO.
We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.
We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects.
We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data.
We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.
NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism.
NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.
NorthEast BioLab truly goes that extra mile, and we hope to continue enjoying seminal interactions with them.
NorthEast BioLab presents a science-based, hands-on, no-frills approach on the latest bioanalytical platforms.
We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.
We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.
Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.
NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.
Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.
This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with top quality and reporting standard with incredible responsiveness.
As a small company, our vendors are key members of our project teams. The scientists at NorthEast BioLab are technical experts, who produce high-quality data, on-time, and on-budget.
At our Mass Spec lab, we offer a wide range of Mass Spectrometry services to enhance your Pharmacokinetic (PK), Toxicology (Tox), Biomarker, Bioavailability (BA), Bioequivalence (BE), DMPK/ADME, Mass Spec Testing, and other research studies.
With state-of-the-art equipment and an experienced team, we guarantee high-quality results and efficient turnaround times. Trust us to accelerate your research studies and provide accurate insights for your project.
Contact us today to learn more about our Mass Spectrometry services and how they can benefit your drug development endeavors.
Experience You Can Trust: With over 20 years of industry-leading experience, our scientists have developed and validated hundreds of LC-MS/MS methods for various applications. From pharmacokinetics to biomarkers, we have worked with diverse biological samples and optimized every process step to ensure reliable and high-quality results. Our expertise spans multiple types of mass spectrometers, guaranteeing the best sensitivity, selectivity, and resolution for your analytes.
Compliance with Confidence: When you choose NorthEast BioLab, you can rest assured that we adhere to the highest regulatory standards. Our scientists follow Good Laboratory Practices (GLP) and Good Clinical Practices (GCP) guidelines, ensuring transparency and traceability in our processes. We understand the requirements of the FDA, EMA, and other regulatory agencies, and we're ready to support you in the submission and approval of your drug applications.
Tailored to Your Needs: At NorthEast BioLab, we prioritize our clients' goals and challenges. Our scientists excel not only in mass spectrometry analysis but also in collaboration and communication. We customize our services to meet your specific needs and expectations. We are flexible and responsive, seeking your approval and feedback at every step. Honesty and transparency are our guiding principles, as we believe in maintaining the highest standards of quality and integrity.
See the Difference: When you choose us for your mass spectrometry data analysis, we guarantee the best service and results for your drug discovery and development projects. Contact us today to learn more about how we can assist you in achieving your research goals.
Mass spectrometry is a widely used procedure observed as having outstanding sensitivity. Triple-quadrupole mass spectrometry (MS/MS) offers additional benefits due to its selectivity. In mass spectrometry analysis, it is essential to isolate….
Mass spectrometry analysis is a powerful tool for determining the molecular weight, sequence, composition, and modifications of various analytes, such as small molecules, proteins, peptides, and oligonucleotides. It can also be combined with other front-end technologies, such as liquid chromatography (LC), gas chromatography (GC), supercritical fluid chromatography (SFC), capillary electrophoresis (CE), and inductively coupled plasma (ICP), to enhance its sensitivity and selectivity.
Electrospray is one of the most common and versatile methods for ionizing analytes, which generates multiple charged ions from a liquid solution. Electrospray can be used for analyzing a wide range of analytes, from small molecules to large biomolecules, and can be coupled with various mass analyzers.
One of the most widely used mass analyzers is the triple quadrupole, which consists of two mass-selective quadrupoles (Q1 and Q3) and a collision cell (Q2) in between. The triple quadrupole can perform multiple reaction monitoring (MRM), a highly selective and sensitive technique for detecting specific analytes. By selecting a parent ion in Q1 and a unique fragment ion in Q3, MRM eliminates the interference from other ions and provides accurate quantification.
Other mass analyzers, such as time-of-flight (TOF), ion trap, or orbitrap, can provide high-resolution and accurate mass measurements. Still, they are less selective and sensitive than the triple quadrupole. They can also be used for analyzing intact proteins or peptides using different fragmentation methods, such as collision-induced dissociation (CID), electron transfer dissociation (ETD), or higher-energy collisional dissociation (HCD), to generate sequence-specificions. Another ionization method, matrix-assisted laser desorption/ionization (MALDI), can also be used for protein or peptide analysis. Unfortunately, it is less compatible with liquid chromatography and produces fewer charge states than electrospray.
Mass spectrometry analysis is a revolutionary tool for research in diverse fields, such as pharmacokinetics, proteomics, metabolomics, and genomics. Generally, electrospray and triple quadrupole mass spectrometry can achieve the best method development, validation, and sample analysis results for the Mass Spectrometry testing services requested by biotech.
Answers to additional Mass Spectrometry Services questions popular among our potential clients.
Mass spectrometry analysis is used in various stages of drug development, from discovery to clinical trials. During the discovery phase, mass spectrometry analysis plays a critical role in drug discovery chemistry when thousands of compounds are tested to select the compound with the highest probability of success in becoming a drug candidate for development.
In the preclinical phase, once drug candidates with good bioavailability are selected, additional dose range finding (DRF) studies are performed in various animal species to enable IND filing. These Tox studies require full toxicokinetic analysis for exact measurement of the drug in rodent and non-rodent species. Mass spectrometry analysis coupled with Liquid chromatography can be used for the analysis of drugs from these studies. Some of the companies that offer Mass Spectrometry services include Charles River, Applied Biomics, and Intertek.
Once the drug moves into the clinical phase, mass spectrometry analysis further helps with pharmacokinetic studies related to NDA filing.
Mass spec proteomics service is a mass spectrometry analysis service that studies proteins and peptides in biological samples. Scientists use mass spec proteomics services to identify proteins, quantify their expression, characterize their modifications, and investigate their interactions and functions. Mass spec proteomics services can provide valuable information for drug development or discovery, disease diagnosis, and biomedical research.
HRMS stands for high-resolution mass spectrometry, a type of mass spectrometry that can measure the mass of ions with very high accuracy and precision, usually in the range of parts per million (ppm) or less. Scientists can use HRMS to determine the exact molecular formula and structure of unknown compounds and distinguish between isomers and isotopes. HRMS can also perform qualitative and quantitative analysis of complex mixtures, such as proteomics and metabolomics samples.
Mass spectrometry is used for diverse purposes, such as: – identifying the elements and isotopes of a sample and determining their precise masses and relative abundances – dating geologic samples by measuring the decay of radioactive isotopes – analyzing inorganic and organic chemicals, especially for small amounts of impurities or contaminants – determining the structural formula and sequence of complex organic molecules, such as peptides, proteins, nucleic acids, and carbohydrates – measuring the strength of chemical bonds and the energy required to produce specific ions – identifying the products of ion decomposition and fragmentation – analyzing unknown materials, such as lunar samples, for their chemical and isotopic composition.
The difference between mass spectrometry and mass spectroscopy is that mass spectrometry is a technique that measures the mass-to-charge ratio of ions. In contrast, mass spectroscopy is a broader term that refers to any method that uses electromagnetic radiation to analyze the properties of matter. Mass spectrometry is a type of mass spectroscopy, but not all mass spectroscopy techniques involve ionization and mass separation. For example, infrared (IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy are also types of mass spectroscopy, but they do not use mass spectrometry.
The specific techniques and methodologies used in mass spectrometry analysis services depend on the type of sample, the research question, and the analytical goal. Some of the standard techniques and methods are: – Electrospray ionization (ESI), which creates a fine spray of charged droplets from a liquid sample using a high voltage – Matrix-assisted laser desorption/ionization (MALDI) that uses a laser to vaporize and ionize molecules embedded in a solid matrix – Gas chromatography-mass spectrometry (GC-MS), which combines a separation technique (GC) with a mass analyzer (MS) to identify and quantify volatile and semi-volatile compounds – Liquid chromatography-mass spectrometry (LC-MS), which combines a separation technique (LC) with a mass analyzer (MS) to identify and quantify polar and non-polar compounds – Tandem mass spectrometry (MS/MS), which uses two or more mass analyzers in sequence to perform fragmentation and structural analysis of ions – Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), which uses a strong magnetic field to trap and measure the frequency of ions with very high resolution and accuracy.
The types of samples or compounds that are typically analyzed using mass spec services are diverse and include: – Biological samples, such as blood, urine, saliva, tissue, cells, proteins, peptides, metabolites, lipids, and nucleic acids – Pharmaceutical samples, such as drugs, drug metabolites, impurities, and excipients – Environmental samples, such as water, soil, air, plants, and animals – Forensic samples, such as explosives, drugs, poisons, and DNA – Industrial samples, such as polymers, catalysts, additives, and contaminants – Archaeological samples, such as artifacts, fossils, and isotopes.
When it comes to protein analysis, mass spectrometry offers an invaluable tool for determining molecular weight, sequence, modifications, and interactions. There are two main approaches for protein analysis: top-down and bottom-up. In top-down analysis, intact proteins are ionized and fragmented in the mass spectrometer, enabling the inference of protein structure and composition from the resulting spectra. In bottom-up analysis, proteins are digested into peptides using enzymes, such as trypsin. The peptides are then separated by liquid chromatography and analyzed by mass spectrometry. The resulting peptide sequences are matched to a protein database to identify the proteins and their modifications.
Peptide analysis, on the other hand, involves determining the molecular weight, sequence, composition, and modifications of peptides. Peptides can be ionized using electrospray or matrix-assisted laser desorption/ionization (MALDI) and analyzed by various types of mass analyzers, such as quadrupole, time-of-flight, ion trap, or orbitrap. Different fragmentation methods, such as collision-induced dissociation (CID), electron transfer dissociation (ETD), or higher-energy collisional dissociation (HCD), are employed to generate sequence-specific ions for peptide identification.
And let's not forget about oligonucleotide analysis. Mass spectrometry is invaluable in determining the molecular weight, sequence, composition, and modifications of
oligonucleotides. These short strands of nucleic acids, such as DNA or RNA, can be synthesized or extracted from biological sources. Oligonucleotides can be ionized using electrospray or MALDI and analyzed by various types of mass analyzers, such as quadrupole, time-of-flight, ion trap, or orbitrap. Oligonucleotide fragmentation can be induced by different methods, such as CID, ETD, or in-source decay (ISD), to generate sequence-specific ions that enable accurate identification of the oligonucleotide and its modifications.
A mass spectrometry service company ensures data accuracy, precision, and quality in their analyses by:
The process of engaging a mass spec service provider usually works as follows:
The types of analytical questions or challenges that scientists can address through mass spectrometry services are manifold and include:
