NorthEast BioLab Logo | Advanced Bioanalytical Services Lab Agile Bioanalytical Services CRO
  • Expertise
    • Menu IconAll Expertise
    • Menu IconMass Spectrometry (LC-MS)
      • Menu IconMass Spectrometry Services
      • Menu IconHPLC Testing Services
    • Menu IconMeso Scale Discovery Assay
    • Menu IconELISA Assay
      • Menu IconLuminex Multiplex Assay
    • Menu IconFlow Cytometry
    • Menu IconqPCR Analysis, ddPCR Assay
    • Menu IconWestern Blot, In Cell Western
  • Services
    • Menu IconAll Services
    • Menu IconPharmacokinetics (PK) Study
      • Menu IconToxicology (Tox) Study
      • Menu IconDose Formulation Analysis
      • Menu IconPK PD Analysis
      • Menu IconDrug Metabolism and Pharmacokinetics (DMPK)
      • Menu IconADME Studies
      • Menu IconBioequivalence and Bioavailability Studies
    • Menu IconADA Assay, Neutralizing Antibody Assay
    • Menu IconMultiplex Cytokine Assay
    • Menu IconBiomarker Assay
      • Menu IconBiomarker Assay List
    • Menu IconCell-Based Assay
      • Menu IconMechanism of Action (MOA) Studies
      • Menu IconCytotoxicity Assay
      • Menu IconBiochemical Assay
    • Menu IconBioanalytical Assay Validation
  • Solutions
    • Menu IconAll Solutions
    • Menu IconClinical CRO
      • Menu IconCentral Lab Services
      • Menu IconPBMC Services
    • Menu IconGLP Laboratory
      • Menu IconBioanalysis
      • Menu IconNon-GLP Testing
    • Menu IconPreclinical CRO
    • Menu IconMethod Development, Validation, And Transfer
      • Menu IconMethod Validation
      • Menu IconMethod Development
      • Menu IconMethod Transfer
    • Menu IconAnalytical Testing
      • Menu IconDiscovery Screening Services
      • Menu IconAnalytical Quantitation
      • Menu IconCosmetic Testing
  • About Us
    • Menu IconAbout Us
    • Menu IconOverview
    • Menu IconLeadership
    • Menu IconFacility and Equipment
    • Menu IconQuality and Compliance
    • Menu IconTherapeutic Areas
    • Menu IconWhy Us?
    • Menu IconValues
    • Menu IconProcess
    • Menu IconPartners
    • Menu IconCareers
      • Business Development Associate
      • Sr. Scientist – Cell Based Assay
      • GLP Quality Assurance Manager
      • Sr. Scientist, ELISA Immunoassay Bioanalysis
      • Sr. Scientist, LC-MS/MS Bioanalysis
      • Associate Scientist, Central Laboratory Services
      • Sr. Scientist - Pharmacokinetics (PK)
      • Content Manager, Scientific and Regulatory Writing
  • Resources
    • Menu IconLearning Center
    • Menu IconBlog
    • Menu IconCase Study
    • Menu IconVideo Brochures
    • Menu IconNews and Events
    • Menu IconTestimonials
    • Menu IconContact Us
  • Request Quote
NorthEast BioLab Logo | Advanced Bioanalytical Services Lab Agile Bioanalytical Services CRO Request Quote

Expertise

  • Menu IconAll Expertise
    • Menu IconMass Spectrometry (LC-MS)
      • Menu IconMass Spectrometry Services
      • Menu IconHPLC Testing Services
    • Menu IconMeso Scale Discovery Assay
      • Menu IconELISA Assay
        • Menu IconLuminex Multiplex Assay
      • Menu IconFlow Cytometry
        • Menu IconqPCR Analysis, ddPCR Assay
          • Menu IconWestern Blot, In Cell Western

            Services

            • Menu IconAll Services
              • Menu IconPharmacokinetics (PK) Study
                • Menu IconToxicology (Tox) Study
                • Menu IconDose Formulation Analysis
                • Menu IconPK PD Analysis
                • Menu IconDrug Metabolism and Pharmacokinetics (DMPK)
                • Menu IconADME Studies
                • Menu IconBioequivalence and Bioavailability Studies
              • Menu IconADA Assay, Neutralizing Antibody Assay
                • Menu IconMultiplex Cytokine Assay
                  • Menu IconBiomarker Assay
                    • Menu IconBiomarker Assay List
                  • Menu IconCell-Based Assay
                    • Menu IconMechanism of Action (MOA) Studies
                    • Menu IconCytotoxicity Assay
                    • Menu IconBiochemical Assay
                  • Menu IconBioanalytical Assay Validation

                    Solutions

                    • Menu IconAll Solutions
                      • Menu IconClinical CRO
                        • Menu IconCentral Lab Services
                        • Menu IconPBMC Services
                      • Menu IconGLP Laboratory
                        • Menu IconBioanalysis
                        • Menu IconNon-GLP Testing
                      • Menu IconPreclinical CRO
                        • Menu IconMethod Development, Validation, And Transfer
                          • Menu IconMethod Validation
                          • Menu IconMethod Development
                          • Menu IconMethod Transfer
                        • Menu IconAnalytical Testing
                          • Menu IconDiscovery Screening Services
                          • Menu IconAnalytical Quantitation
                          • Menu IconCosmetic Testing

                        About Us

                        • Menu IconAbout Us
                          • Menu IconOverview
                            • Menu IconLeadership
                              • Menu IconFacility and Equipment
                                • Menu IconQuality and Compliance
                                  • Menu IconTherapeutic Areas
                                    • Menu IconWhy Us?
                                      • Menu IconValues
                                        • Menu IconProcess
                                          • Menu IconPartners
                                            • Menu IconCareers
                                              • Menu IconBusiness Development Associate
                                              • Menu IconSr. Scientist – Cell Based Assay
                                              • Menu IconGLP Quality Assurance Manager
                                              • Menu IconSr. Scientist, ELISA Immunoassay Bioanalysis
                                              • Menu IconSr. Scientist, LC-MS/MS Bioanalysis
                                              • Menu IconAssociate Scientist, Central Laboratory Services
                                              • Menu IconSr. Scientist - Pharmacokinetics (PK)
                                              • Menu IconContent Manager, Scientific and Regulatory Writing

                                            Resources

                                            • Menu IconLearning Center
                                              • Menu IconBlog
                                                • Menu IconCase Study
                                                  • Menu IconVideo Brochures
                                                    • Menu IconNews and Events
                                                      • Menu IconTestimonials
                                                        • Menu IconContact Us

                                                          Request Quote

                                                            Contact Us

                                                            NorthEast BioLab Logo Agile Bioanalysis Services CRO
                                                            Request Quote Contact Us
                                                            • Home
                                                            • Cell Cytotoxicity Assay, Cell Toxicity Assay, Cytotoxicity Screening Services Lab CRO

                                                            Expedite Your Therepautic’s Development With Our In-vitro, Cell Cytotoxicity Assay And Cell Toxicity Assay Services

                                                            • Rapid, Quantitative Drug-Induced Cell Toxicity Assay Services, Including Cell Viability, Proliferation, Apoptosis, Necrosis, And Inflammation
                                                            • Characterizing Pharmacological Properties Of Therapeutic Compounds To Evaluate Efficacy, Safety, Toxicity, Etc.
                                                            • Establish Mammalian Cell Line Culture Utilizing Transfection, Viral Infection, Gene Editing, And Cell Selection (100+ Cell Lines Maintained)
                                                            • Expert Guidance And Assistance For In Vitro And In-Vivo Study Assay Design, Sample Collection, And Cytotoxicity Testing Services

                                                            Regulated And Inspected By:

                                                            Good Laboratory Practice for Nonclinical Laboratory Studies | NorthEast BioLab Food and Drug Administration (FDA | NorthEast BioLab Clinical Laboratory Improvement Amendments | NorthEast BioLab International Orgaization for Standardization |NorthEast BioLab

                                                            Provide Your Bioanalysis Study Details

                                                            We Will Respond Within 1 Business Day

                                                            Cell Cytotoxicity | NorthEast BioLab
                                                            Click below for answers to your Cytotoxicity Assay questions -
                                                            • What is Cytotoxicity Assay?
                                                              What is Cytotoxicity Assay?
                                                            • What are the various Cell Cytotoxicity Assay Testing Services Offered by Us?
                                                              What are the various Cell Cytotoxicity Assay Testing Services Offered by Us?
                                                            • Why Choose NorthEast BioLab for Your Cytotoxicity Testing?
                                                              Why Choose NorthEast BioLab for Your Cytotoxicity Testing?
                                                            • What is the Role of Cytotoxicity Assay during Drug Development?
                                                              What is the Role of Cytotoxicity Assay during Drug Development?

                                                            Ask Us About Your Study

                                                            In-vitro Cytotoxicity and Cell Viability Assays based on cellular function and activity for Drug Discovery and Fundamental Research

                                                            SPEAK TO OUR SCIENTISTS

                                                            What is Cytotoxicity Assay?

                                                            Cytotoxicity Assay: Evaluating Drug Safety and Cell Proliferation

                                                            Cytotoxicity assays are developed to analyze the ability of certain drug compounds to destroy the healthy cells of an organism. Cytotoxic compounds can lead to accidental cell death (referred to as necrosis) or programmed cell death (referred to as apoptosis). During the nonclinical and clinical research phases of drug discovery and development, cytotoxicity assays assess the safety profile of the drug candidate. The method proves valuable in identifying off-target effects of certain drug compounds on the human body.

                                                            For drug compounds developed for cancer treatment, cytotoxicity assays are critical in a variety of testing activity. Compounds that can hinder or stop cell proliferation make strong anti-cancer candidates.

                                                            Multiple cytotoxicity assays are used to assess different cell functions such as cell membrane permeability, enzyme activity, ATP production, cell adherence, nucleotide uptake, and co-enzyme production. Cell toxicity assay should be selected based on the drug compound and with careful consideration of cellular function. The most effective method for cytotoxicity screening of the drug compound is evaluating cell membrane integrity. Cells having cytotoxic effects often show signs of compromised membrane integrity.

                                                            Sponsors Love Our Bioanalytical Laboratory Services For Top Quality And Industry Leading Turnaround

                                                            Connect With Us For A Reference Call With Them!

                                                            User Vector
                                                            VP, Bioanalytical Development

                                                            “NorthEast BioLab is the most responsive and thorough bioanalysis lab services CRO.”

                                                            User Vector
                                                            VP, Biomarker Development 

                                                            NorthEast BioLab is a responsive, collaborative, and reliable partner

                                                            User Vector
                                                            Executive Director, Pharmacokinetics

                                                            We trust NorthEast BioLab to design and execute streamlined, impactful bioanalytical projects

                                                            User Vector
                                                            President & CSO, Biotech

                                                            We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data

                                                            User Vector
                                                            Sr. Associate Dir., Clinical Trials

                                                            We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

                                                            User Vector
                                                            Co-Founder, Biotech & University PI

                                                            NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism

                                                            User Vector
                                                            VP, PK PD Analysis

                                                            NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

                                                            User Vector
                                                            Head, Bioanalytical Development

                                                            “NorthEast BioLab goes the extra mile. We look forward to more meaningful collaborations.”

                                                            User Vector
                                                            Sr. Dir., Bioanalytical Development & QC

                                                            NorthEast BioLab offers a science-based, hands-on approach to the latest bioanalytical platforms

                                                            User Vector
                                                            SVP Quality & Compliance, Biotech

                                                            We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

                                                            User Vector
                                                            Director, PK

                                                            We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

                                                            User Vector
                                                            VP, Head of Clinical Operations

                                                            Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

                                                            User Vector
                                                            Executive Director, Drug Research

                                                            NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

                                                            User Vector
                                                            Dir., Bioequivalence Testing

                                                            Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

                                                            User Vector
                                                            Head, PK

                                                            This study, same as all other bioanalytical studies, was completed with top quality and reporting standard with incredible responsiveness.

                                                            User Vector
                                                            VP, Development Operations

                                                            NorthEast BioLab’s scientists deliver high-quality data on time and within budget

                                                            Tackle Your Cell Cytotoxicity Assay With Our Veteran Team Now!

                                                            SPEAK TO OUR SCIENTISTS

                                                            What are the various Cytotoxicity Assay Testing Services Offered by Us?

                                                            Cytotoxicity Assay Testing Services by NorthEast BioLab

                                                            Cell cytotoxicity assays are utilized during drug development before toxicological testing is performed. Cytotoxicity assays are additionally used for controlling the quality of manufactured drug compounds.

                                                            There are quantitative and qualitative methods of cytotoxicity testing. The quantitative cytotoxicity assays used by NorthEast BioLab are Luciferase-ATP assay and MTT assay, and the qualitative assays used are MEM elution, the direct contact test, and the agar diffusion test.

                                                            Quantitative Assays

                                                            Luciferase-ATP Assay – Cell viability is an alternate readout for cytotoxicity. Luciferase is a commonly used enzyme that converts luciferin to oxyluciferin in the presence of ATP. ATP is found in viable cells and is proportional to the number of viable cells present. This assay is adaptable to high-throughput formats. It is also a sensitive readout of cytotoxicity in a variety of cells.

                                                            MTT Assay – The MTT cytotoxicity assay uses 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye, commonly referred to as MTT. MTT is a yellow-colored water-soluble compound which is split by mitochondrial succinate dehydrogenase, giving rise to the violet-colored formazan. The conversion of MTT to formazan only occurs in viable cells.

                                                            Qualitative Assays

                                                            Qualitative assays, such as the MEM elution assay, direct contact test, and agar diffusion test are used infrequently. In these assays, cells are exposed to a drug or compound, and cell deformity is assessed visually, on a predetermined scale.

                                                            Cytotoxicity Assay Testing | NorthEast BioLab

                                                            Registered And Inspected By Various Regulatory Agencies

                                                            • Good Laboratory Practice for Nonclinical Laboratory Studies | NorthEast BioLab
                                                            • Food and Drug Administration (FDA | NorthEast BioLab
                                                            • clia
                                                            • United States Drug Enforcement Administration | NorthEast BioLab
                                                            • Department of Consumer Protection | NorthEast BioLab

                                                            Don’t Wait, Start Your Cell Toxicity Assay Service Today!

                                                            SPEAK TO OUR SCIENTISTS
                                                            Cytotoxicity Testing to Achieve Accurate Detection and Quantitation during Drug R&D | NorthEast BioLab

                                                            Why Choose NorthEast BioLab for Your Cytotoxicity Testing?

                                                            Tailored Cytotoxicity Testing to Achieve Accurate Detection and Quantitation during Drug R&D

                                                            NorthEast BioLab uses cytotoxicity screening as a marker for biocompatibility of the drug compounds or selected drug candidates. We perform these cytotoxicity tests as per our documented SOPs to ensure adequate safety screening of various drug compounds.

                                                            When the cell toxicity assay result of a sample compound is unfavorable, or the toxicity of the compound is beyond an acceptable range, the drug candidate cannot proceed to the next stage of drug development. In this situation, our team of scientists conducts a toxicity risk assessment to determine the mechanism of toxicity and identify potential pathways to mitigate the toxic effects of the drug candidate.

                                                            NorthEast BioLab understands the importance of cytotoxicity testing. Our scientists follow FDA guidelines to decide whether to proceed with a compound safely. We partner with clients to ensure that your final drug product is safe for the human cells.

                                                            The Basics of Bioanalysis

                                                            Bioanalysis is an essential tool in drug discovery and development for determining the concentration of drugs and their metabolites as well as various pharmacodynamics biomarkers in biological fluids. In these analyses, scientists use developed an….

                                                            Read More
                                                            cytotoxicity video
                                                            Basics Of Bioanalysis | NorthEast BioLab
                                                            Cytotoxicity Assay from Drug Discovery and Preclinical to Clinical Phases | NorthEast BioLab

                                                            What is the Role of Cytotoxicity Assay during Drug Development?

                                                            Cytotoxicity Assay: From Drug Discovery and Preclinical to Clinical Phases

                                                            Every new drug candidate is continuously subjected to extensive tests for safety and efficacy before approval. Cytotoxicity screening is one way to test the safety of a drug on target and off-target cells in early stages of drug development. Toxicity testing for on- and off-target effects is necessary for safe drug administration.

                                                            In vitro assessments establish the toxicity of a drug compound in the human body. The permeability, metabolic stability, and interactions to membrane transporter systems are assessed in cell models relevant to the human body. A safety profile is achieved by combining cell toxicity assay testing with in vivo tests of absorption, distribution, metabolism, and excretion.

                                                            Although cytotoxicity assays aim at finding toxicity of the drug compound or sample compound, it is unlikely to assess toxicity with a single test accurately. Multiple cytotoxicity screening models are used for cell viability assessments in various human cell lines.

                                                            Trust Our Cytotoxicity Screening Services Lab CRO.

                                                            SPEAK TO OUR SCIENTISTS

                                                            Explore More Services

                                                            • Nonclinical Toxicology (Tox) Studies | NorthEast BioLab Toxicology (Tox)
                                                              Study
                                                            • Dose Formulation | NorthEast BioLab Dose Formulation
                                                              Analysis
                                                            • PK PD Assay And Analysis | NorthEast BioLab PK PD
                                                              Analysis
                                                            • Drug Metabolism and Pharmacokinetics Lab | NorthEast BioLab Drug Metabolism and Pharmacokinetics (DMPK)
                                                            • ADME Lab | NorthEast BioLab ADME
                                                              Studies
                                                            • Bioequivalence (BE) Studies Testing | NorthEast BioLab Bioequivalence and Bioavailability Studies
                                                            • Mechanism Of Action Study | NorthEast BioLab Mechanism of Action (MOA) Studies
                                                            • Cell Cytotoxicity | NorthEast BioLab Cytotoxicity
                                                              Assay
                                                            • Biochemical Test Lab | NorthEast BioLab Biochemical
                                                              Assay

                                                            Contact A Scientist With Your Bioanalytical Study Details

                                                            We Will Respond Within 1 Business Day

                                                            Expertise

                                                            Expertise

                                                            • Mass Spectrometry (LC-MS)
                                                            • Meso Scale Discovery Assay
                                                            • ELISA Assay
                                                            • Flow Cytometry
                                                            • qPCR Analysis, ddPCR Assay
                                                            • Western Blot, In Cell Western

                                                            Services

                                                            Services

                                                            • Pharmacokinetics (PK) Study
                                                            • ADA Assay, Neutralizing Antibody Assay
                                                            • Multiplex Cytokine Assay
                                                            • Biomarker Assay
                                                            • Cell-Based Assay
                                                            • Bioanalytical Assay Validation

                                                            Solutions

                                                            Solutions

                                                            • Clinical CRO
                                                            • GLP Laboratory
                                                            • Preclinical CRO
                                                            • Method Development, Validation, And Transfer
                                                            • Analytical Testing

                                                            About Us

                                                            About Us

                                                            • Overview
                                                            • Leadership
                                                            • Facility and Equipment
                                                            • Quality and Compliance
                                                            • Therapeutic Areas
                                                            • Why Us?
                                                            • Values
                                                            • Process
                                                            • Partners
                                                            • Careers

                                                            Resources

                                                            Resources

                                                            • Learning Center
                                                            • Blog
                                                            • Case Study
                                                            • Video Brochures
                                                            • News and Events
                                                            • Testimonials
                                                            • Contact Us
                                                            NorthEast BioLab Logo Agile Bioanalytical Services CRO
                                                            sales@nebiolab.comFollow Up On Email
                                                            (203) 361 3780Follow Up On Phone

                                                            35 Worth Ave, Hamden, CT 06518

                                                            • Good Laboratory Practice for Nonclinical Laboratory Studies | NorthEast BioLab
                                                            • Food and Drug Administration (FDA | NorthEast BioLab
                                                            • clia
                                                            • United States Drug Enforcement Administration | NorthEast BioLab
                                                            • Department of Consumer Protection | NorthEast BioLab
                                                            • Privacy Policy
                                                            • Terms of Use
                                                            • Sitemap
                                                            All rights reserved.