What are the various Cytotoxicity Assay Testing Services Offered by Us?
Cytotoxicity Assay Testing Services by NorthEast BioLab
Cytotoxicity assays are utilized during drug development before toxicological testing is performed. Cytotoxicity assays are additionally used for controlling the quality of manufactured drug compounds.
There are quantitative and qualitative methods of cytotoxicity testing. The quantitative cytotoxicity assays used by NorthEast BioLab are Luciferase-ATP assay and MTT assay, and the qualitative assays used are MEM elution, the direct contact test, and the agar diffusion test.
Luciferase-ATP Assay – Cell viability is an alternate readout for cytotoxicity. Luciferase is a commonly used enzyme that converts luciferin to oxyluciferin in the presence of ATP. ATP is found in viable cells and is proportional to the number of viable cells present. This assay is adaptable to high-throughput formats. It is also a sensitive readout of cytotoxicity in a variety of cells.
MTT Assay – The MTT cytotoxicity assay uses 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye, commonly referred to as MTT. MTT is a yellow-colored water-soluble compound which is split by mitochondrial succinate dehydrogenase, giving rise to the violet-colored formazan. The conversion of MTT to formazan only occurs in viable cells.
Qualitative assays, such as the MEM elution assay, direct contact test, and agar diffusion test are used infrequently. In these assays, cells are exposed to a drug or compound, and cell deformity is assessed visually, on a predetermined scale.