How do we perform MIF lab test?
Protocols we follow to perform MIF tests
The MIF gene encodes MIF cytokine to escalate an immune response. Through MSD, ELISA, and Luminex assays, we at NorthEast BioLabs detect MIF in your samples. We can detect MIF in human plasma, serum, and cell culture supernatants. We briefly describe on our ELISA protocol in the following section.
We begin with 100 μl of sample or standard solution in each well precoated with antibodies against MIF and incubate them for 2.5 hours at room temperature or O/N at 4℃. We then incubate the wells with 100 μl of biotinylated detection antibody for 1 hr at RT. Post incubation, we add 100 μl of streptavidin-conjugated HRP solution in each well and incubate it for 45 mins at RT. We then introduce a TMB one-step substrate solution in each well and incubate it at RT. Finally, 50 μl of stop solution is added to end of the reaction and immediately read on a spectrophotometer at 450 nm.
The macrophage migration inhibitory factor ELISA assay or MIF test is a quantitative, colorimetric, sandwich-based assay technique. The assay can detect between 6 pg/ml to 6000 pg/ml, with the minimum detection dose of 6 pg/ml.