VP, Bioanalytical Development
- Expertise
- Services
- Solutions
- About Us
- Resources
Expertise
Services
Solutions
About Us
About Us- Overview
- Leadership
- Facility and Equipment
- Quality and Compliance
- Therapeutic Areas
- Why Us?
- Values
- Process
- Partners
- Careers
Business Development Associate
Sr. Scientist – Cell Based Assay
GLP Quality Assurance Manager
Sr. Scientist, ELISA Immunoassay Bioanalysis
Sr. Scientist, LC-MS/MS Bioanalysis
Associate Scientist, Central Laboratory Services
Sr. Scientist - Pharmacokinetics (PK)
Content Manager, Scientific and Regulatory Writing
Resources
Glial fibrillary acidic protein
- Glial fibrillary acidic protein/GFAP is a type III intermediate filament protein
- GFAP function is not fully understood but is highly expressed in CNS tissues
- GFAP may serve as a biomarker of CNS disorders including Alexander disease
- GFAP can be assayed using immunoreactive based assays: ELISA, MSD or Luminex
Regulated And Inspected By:
Provide Your Bioanalysis Study Details
We Will Respond Within 1 Business Day
Click below for answers to your GFAP Biomarker Study questions -
What is GFAP?
An Introduction to Glial fibrillary acidic protein (GFAP)
Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein belonging to the intermediate filament protein family. The GFAP gene in humans encodes the GFAP protein. The gene is located on chromosome 17. Several CNS cell types that include ependymal cells and astrocytes express GFAP proteins during development. Other human cells, such as keratinocytes, Leydig cells, chondrocytes, and osteocytes, also express GFAP. GFAP, along with the other three non-epithelial cells belonging to the same protein family, regulates the functions and structure of the cytoskeleton. Although many studies use GFAP as a cell marker, we have still not completely understood its role in the body.
Studies suggest a GFAP protein can help maintain cell shape and astrocyte mechanical strength. Type III intermediate filament proteins comprise three domains, head, rod, and tail. The rod domain may differ in its DNA sequence, but its protein structure is highly conserved. GFAP can form both a homodimer and heterodimer and can polymerize other proteins in the type III group. GFAP comprises eight different isoforms that make up distinct astrocyte subpopulations in the human brain. Out of these isoforms, GFAP alpha is the only one that can assemble homomerically. Another isoform, GFAP delta, is one of the most researched GFAP isoforms. Studies have linked GFAP delta isoform with neural stem cells.
You can also use the other names like GFAP and ALXDRD when searching for ‘What is GFAP?’ or ‘What is Glial fibrillary acidic protein’?
Sponsors Love Our Bioanalytical Laboratory Services For Top Quality And Industry Leading Turnaround
Connect With Us For A Reference Call With Them!
NorthEast BioLab is a responsive, collaborative, and reliable partner
We trust NorthEast BioLab to design and execute streamlined, impactful bioanalytical projects
We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data
We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.
NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism
NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.
“NorthEast BioLab goes the extra mile. We look forward to more meaningful collaborations.”
NorthEast BioLab offers a science-based, hands-on approach to the latest bioanalytical platforms
We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.
We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.
Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.
NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.
Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.
This study, same as all other bioanalytical studies, was completed with top quality and reporting standard with incredible responsiveness.
NorthEast BioLab’s scientists deliver high-quality data on time and within budget
Tackle Your GFAP Biomarker Assay Testing With Our Veteran Team Now!
How do we perform GFAP lab test?
Protocols we follow to perform GFAP tests
We perform MSD, Luminex, and ELISA assays for detecting GFAP biomarker in plasma, serum, and other biological fluids. Following is a Sandwich-based ELISA protocol used to detect GFAP protein in any biological fluids.
We begin the GFAP test with a microtiter plate, pre-coated with a GFAP-specific antibody. We then add 100 μl of standard or samples, followed by a GFAP specific biotin-conjugated antibody. Next, we incubate the wells with Avidin-conjugated HRP. We then add a TMB substrate solution in each well. The wells containing all three biotin-conjugated antibodies, GFAP, and enzyme-conjugated Avidin exhibit a change in color. Lastly, we end the reaction by adding a sulphuric acid solution and measure the color change spectrophotometrically at 450 nm (± 10 nm). We then compare the Optical Density (OD) with the standard curve and determine GFAP concentration.
The assay for GFAP immunoreactivity has a detection range of 31.2 pg/ml to 2000 pg/ml with a minimum detectable dose of < 10.2 pg/ml. The GFAP assay is highly specific, with an intra-assay precision of < 10% and inter-assay precision of <12%.
Registered And Inspected By Various Regulatory Agencies
Don’t Wait, Start Your GFAP Biomarker Assay Testing Today!
Where could I learn more about GFAP Assay?
Popular GFAP resources for you to explore
Studies have associated improper GFAP regulation with several disorders. GFAP dysregulation induces glial cells to cause detrimental injuries. Post-injury, the cells upregulate GFAP protein levels, and they interact with astrocytes and fibrous tissues to form a scar that re-establishes the glial margins. Alexander disease is another condition directly related to the presence of the GFAP. Alexander disease is a rare genetic disorder with symptoms including dementia, mental and physical retardation, seizures, and head and brain size enlargements.
To learn more about the biological role of GFAP and GFAP immunoreactivity, explore the resources below.
https://ghr.nlm.nih.gov/gene/GFAP
https://www.genecards.org/cgi-bin/carddisp.pl?gene=GFAP
https://www.uniprot.org/uniprot/P14136
https://www.omim.org/entry/137780
https://www.sciencedirect.com/topics/neuroscience/glial-fibrillary-acidic-protein
Multiplex Cytokine Analysis, MSD Cytokine Assay
Understand Multiplex Cytokine Analysis, Meso Scale Discovery (MSD) Cytokine Assay For Inflammation, Chemokines, Interleukins, Growth Factor …
What are GFAP Functions and Effects?
Understanding the functions and effects of GFAP
As astrocytes express GFAP, it regulates crucial CNS processes that include cellular communication and efficient functioning of the blood-brain barrier. Studies have shown that the GFAP gene adjusts its filament network and regulates mitosis in the cell. Studies of the GFAP biomarker in knockout mice induce white matter structure deterioration, abnormal myelination, and deterioration of the blood-brain barrier. On the other hand, in GFAP knockout mice, Purkinje cells were unable to show their original structure, and these mice perform inefficiently in conditioning experiments. In vitro studies that use antisense RNA show that astrocytes lacking GFAP protein are incapable of forming normal neuronal extensions. GFAP hence, plays a critical role in CNS injury repair, especially to form glial scars throughout the CNS.
Trust Our GFAP Biomarker Assay Testing.
Explore More Biomarkers
-
Brain-Derived Neurotrophic Factor
-
Granulate Colony Stimulation Factor
-
Epidermal growth factor
-
Fas Ligand
-
Granulocyte Macrophage Colony Stimulating Factor
-
Leukemia Inhibitory Factor
-
Luteinizing hormone
-
Macrophage Migration Inhibitory Factor
-
Interferon Beta
-
Interferon Gamma
-
Transforming Growth Factor Beta 1
-
B Type Natriuretic Peptide
-
Glial Fibrillary Acidic Protein
-
Cystatin C
-
Hepatocyte Growth Factor
-
Interferon Alpha 2
-
Tumor Necrosis Factor Alpha
