sales@nebiolab.com Email Your Inquiry
(203) 361 3780 Speak to Our Scientists
Agile Bioanalysis Services CRO
  • Expertise
    • Liquid Chromatography Mass Spectrometry
      • HPLC Method Development Validation
      • Mass Spectrometric Analysis
    • ELISA Method
    • MSD Assay
    • Luminex Multiplex Assay
    • LI-COR Odyssey Assay
    • PK PD Analysis
    • Method Development, Validation, and Transfer
      • Method Validation
      • Method Development
      • Method Transfer
  • Services
    • Pharmacokinetics (PK) Study
      • Drug Metabolism and Pharmacokinetics (DMPK)
      • ADME Studies
    • Toxicology (Tox) Study
      • Dose Formulation Analysis
    • Biomarker Testing Services
      • Biomarker Assay List
    • Bioequivalence and Bioavailability Studies
    • Immunogenicity Testing
    • Bioanalytical Assay
      • Cell-based Assays
      • Cytotoxicity Assay
      • Biochemical Assay
      • Mechanism of Action (MOA) Studies
  • Solutions
    • Bioanalysis
    • GLP Laboratory
    • Clinical CRO
      • Clinical Trial Sample Management and Storage
    • Preclinical CRO
    • Analytical Testing
      • Analytical Quantitation
    • Non-GLP Testing
      • Discovery and Research
      • Cosmetic Testing
  • About Us
    • Overview
    • Why Us?
    • Values
    • Leadership
    • Process
    • Quality and Compliance
    • Facility and Equipment
    • Therapeutic Areas
    • Partners
    • Careers
  • Resources
    • Learning Center
    • Blog
    • Case Study
    • News and Events
    • Testimonials
    • Contact Us
  • Request Quote
Agile Bioanalysis Services CRO Request Quote

Expertise

  • Liquid Chromatography Mass Spectrometry
    • HPLC Method Development Validation
    • Mass Spectrometric Analysis
  • ELISA Method
    • MSD Assay
      • Luminex Multiplex Assay
        • LI-COR Odyssey Assay
          • PK PD Analysis
            • Method Development, Validation, and Transfer
              • Method Validation
              • Method Development
              • Method Transfer

            Services

            • Pharmacokinetics (PK) Study
              • Drug Metabolism and Pharmacokinetics (DMPK)
              • ADME Studies
            • Toxicology (Tox) Study
              • Dose Formulation Analysis
            • Biomarker Testing Services
              • Biomarker Assay List
            • Bioequivalence and Bioavailability Studies
              • Immunogenicity Testing
                • Bioanalytical Assay
                  • Cell-based Assays
                  • Cytotoxicity Assay
                  • Biochemical Assay
                  • Mechanism of Action (MOA) Studies

                Solutions

                • Bioanalysis
                  • GLP Laboratory
                    • Clinical CRO
                      • Clinical Trial Sample Management and Storage
                    • Preclinical CRO
                      • Analytical Testing
                        • Analytical Quantitation
                      • Non-GLP Testing
                        • Discovery and Research
                        • Cosmetic Testing

                      About Us

                      • Overview
                        • Why Us?
                          • Values
                            • Leadership
                              • Process
                                • Quality and Compliance
                                  • Facility and Equipment
                                    • Therapeutic Areas
                                      • Partners
                                        • Careers

                                          Resources

                                          • Learning Center
                                            • Blog
                                              • Case Study
                                                • News and Events
                                                  • Testimonials
                                                    • Contact Us

                                                      Request Quote

                                                        Contact Us

                                                        NorthEast BioLab Logo Agile Bioanalysis Services CRO
                                                        Request Quote Contact Us
                                                        • Home
                                                        • TNFα Biomarker Assay Testing

                                                        Tumor necrosis factor-alpha

                                                        • Tumor necrosis factor-alpha/ TNFα is an adipokine and a cytokine belonging to TNF superfamily
                                                        • TNFα is produced by immune cells
                                                        • TNFα is a biomarker of systemic inflammation
                                                        • TNFα can be assayed using ELISA, MSD or Luminex
                                                        Good Laboratory Practice for Nonclinical Laboratory StudiesFood and Drug Administration (FDA)

                                                        Provide Your Bioanalysis Study Details

                                                        We Will Contact You Within 1 Business Day

                                                        Click below for answers to your TNFα Biomarker Study questions -
                                                        • What is TNFα?
                                                          What is TNFα?
                                                        • What are TNFα Functions and Effects?
                                                          What are TNFα Functions and Effects?
                                                        • How do we perform TNFα lab test?
                                                          How do we perform TNFα lab test?
                                                        • Where could I learn more about TNFα Assay?
                                                          Where could I learn more about TNFα Assay?

                                                        What is TNFα?

                                                        An Introduction to Tumor Necrosis Factor Alpha (TNFα)

                                                        Tumor necrosis factor-alpha, TNFα, is a human cytokine associated with systemic inflammation and is an integral component of the acute phase reaction. Primarily, activated macrophages produce TNFα, but other cell types such as natural killer cells, mast cells, T helper cells, eosinophils, and neutrophils can also produce TNFα. These cells produce TNFα as a stable 233 amino acid long homotrimer protein, from which a metalloprotease, TNFα-converting enzyme proteolytically cleaves and releases a soluble homotrimeric cytokine.

                                                        TNFα belongs to the TNF superfamily that comprises several transmembrane proteins. The human tumor necrosis factor alpha primarily regulates the immune cells. TNFα is an endogenous pyrogen and therefore, can induce fever, inflammation, and apoptotic cell death. TNFα can inhibit viral replication, oncogenesis, and along with interleukin (IL)-1 and IL-6 cells, it can also respond to sepsis.

                                                        When you browse for ‘What is Tumor Factor Necrosis Alpha’ online, you will get results for tumor necrosis factor (TNF), cachectin and cachexin.

                                                        What Our Sponsors Have to Say?

                                                        VP, Bioanalytical Development

                                                        NorthEast BioLab is by far the most responsive and thorough bioanalysis lab services CRO.

                                                        VP, Biomarker Development 

                                                        We partnered on several programs and found NorthEast BioLab responsive, collaborative, and reliable.

                                                        Executive Director, Pharmacokinetics

                                                        We trust NorthEast BioLab to design and execute the most streamlined and impactful bioanalytical projects.

                                                        President & CSO, Biotech

                                                        We found their integrity as refreshing as readiness to provide creative scientific input and high-quality data.

                                                        Sr. Associate Director, Clinical Trials

                                                        We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data.

                                                        Co-Founder, Biotech & University PI

                                                        NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism.

                                                        VP, PK PD Analysis

                                                        NorthEast BioLab always exceeds expectations on bioanalytical assay development, validation, and sample analysis.

                                                        Head, Bioanalytical Development

                                                        NorthEast BioLab truly goes that extra mile, and we hope to continue enjoying seminal interactions with them.

                                                        Sr.Director, Bioanalytical Development & QC

                                                        NorthEast BioLab presents a science-based, hands-on, no-frills approach on the latest bioanalytical platforms.

                                                        SVP Quality & Compliance, Biotech

                                                        We are thrilled to complete our bioanalytical studies with their top quality and incredibly responsive team.

                                                        Director, PK

                                                        We worked closely to implement the most efficient and cost-effective bioanalytical assay for our PK Studies.

                                                        VP, Head of Clinical Operations

                                                        Our projects with NorthEast BioLab include successful method development, validation, stability studies during Clinical Phase I – IV studies.

                                                        Executive Director, Drug Research

                                                        NorthEast BioLab provides critical insight, and are compliant with regulatory standards and industry best practices. We highly recommend them and look forward to working together again.

                                                        Director, Bioequivalence Testing

                                                        Our latest successful study was a pivotal bioequivalence study, where samples from a cross-over study with about 100 volunteers needed swift analysis.

                                                        Head, PK

                                                        This study, same as all other bioanalytical studies with NorthEast BioLab, was completed with top quality and reporting standard with incredible responsiveness.

                                                        VP, Development Operations

                                                        As a small company, our vendors are key members of our project teams. The scientists at NorthEast BioLab are technical experts, who produce high-quality data, on-time, and on-budget.

                                                        Ready to Take the First Step?

                                                        Consult with a Scientist About Your TNFα Biomarker Assay Study
                                                        SPEAK TO OUR SCIENTISTS
                                                        EGF Functions and Effects

                                                        What are TNFα Functions and Effects?

                                                        Understanding the functions and effects of TNFα

                                                        How do you define tumor necrosis factor? Tumor necrosis factor alpha or TNFα binds to two receptors, TNFR1 and TNFR2. Most tissues express the TNFR1 receptor, and both the soluble trimeric form and membrane-bound TNFα can activate the TNFR1 receptor. On the other hand, the immune system expresses the TNFR2 receptor, and the membrane-bound TNFα homotrimer activates the TNFR2 receptor. TNFα combined with IL-1 and IL-6 performs a broad spectrum of biological activity. TNFα can trigger the release of the corticotropin-releasing hormone and stimulate the hypothalamic-pituitary-adrenal axis. TNFα can stimulate the acute phase response in the liver and cause an increase in C-reactive protein levels. They can also promote serine-phosphorylation of IRS-q and induce insulin resistance.

                                                        TNFα is a potent neutrophil attractant. Tumor necrosis factor alpha function is to promote adhesion molecule expression and facilitate neutrophil migration. A local increase in TNF levels induces symptoms of inflammation. While high concentrations may induce shock-like symptoms, prolonged low TNF levels can cause cachexia.

                                                        Drug Discovery And Development Process

                                                        Drug Discovery and Development Process

                                                        How long do you think it takes for a new drug to get approved in the US? About 12-15 years, that’s roughly 1/3rd duration of the entire professional career for any of us. Next, how much investment do you think is required to bring a new drug to market?

                                                        Read More at our Learning Center

                                                        How do we perform TNFα lab test?

                                                        Protocols we follow to perform TNFα tests

                                                        At NorthEast BioLabs, we use MSD, ELISA, and Luminex assays to detect tumor necrosis factor alpha (TNFα) in human plasma or serum. Following describes a Luminex protocol followed for the tumor necrosis factor alpha assay.

                                                        We begin with 200 μl of assay buffer in each well, shake it for 10 min at room temperature (RT), and decant it. Once we remove all the residues, we add 25 μl of the following components in appropriate wells: standard, control or samples, followed by assay buffer, serum matrix, and beads and are incubated overnight at 4 ℃. Once the beads capture an analyte, we add 50 μl of biotinylated detection antibody and incubate it for 1 hr at RT. We then incubate the wells with a 50 μl streptavidin-phycoerythrin, a reporter molecule to end the surface reaction. Last, we add 100 μl of the sheath or drive fluid and read the plate on a Luminex platform.

                                                        The assay is highly sensitive (0.3 pg/ml) with an intra-assay precision of < 10% and an inter-assay precision of < 20%. The assay is highly accurate with a 91% recovery in the serum matrix.

                                                        EGF lab test

                                                        Registered And Inspected By Various Regulatory Agencies

                                                        learn more about EGF Assay

                                                        Where could I learn more about TNFα Assay ?

                                                        Popular TNFα resources for you to explore

                                                        Tumor necrosis factor alpha or TNFα induces inflammatory responses in humans. The inflammatory responses activated are critical in several clinical conditions associated with autoimmune disorders. TNF inhibitors bind with greater affinity to TNFα and inhibit its tumor necrosis factor alpha function or activation. Some examples of TNF inhibitors are infliximab, adalimumab, certolizumab pegol, and etanercept. We should note that in some patients, TNF inhibitors might aggravate their disease condition or facilitate the onset of new autoimmunity.

                                                        Anti-TNF therapy has delivered average results for cancer treatment. Studies have shown that infliximab treatment in certain renal cell carcinoma patients caused prolonged stabilization of the disease. Similarly, etanercept treatment in breast and ovarian cancer patients showed prolonged stabilization of the disease via downregulation. On the other hand, studies combining infliximab or etanercept with gemcitabine for treatment of advanced pancreatic cancer could not show any efficacy in outcome when compared with the placebo group.

                                                        To learn more about the biological activity of TNFα and its detection assays, explore the resources below.
                                                        https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631033/
                                                        https://www.webmd.com/rheumatoid-arthritis/how-does-tnf-cause-inflammation
                                                        https://www.sciencedirect.com/topics/medicine-and-dentistry/tumor-necrosis-factor
                                                        https://www.medicalnewstoday.com/articles/324841
                                                        https://www.sinobiological.com/resource/cytokines/what-is-tnf

                                                        Still Have Questions Regarding Your TNFα Biomarker Assay Testing?

                                                        The Scientists At NorthEast BioLab are Ready to Help!
                                                        SPEAK TO OUR SCIENTISTS

                                                        Explore More Biomarkers

                                                        • Brain-Derived Neurotrophic Factor Brain-Derived Neurotrophic Factor
                                                        • Granulate Colony Stimulation Factor Granulate Colony Stimulation Factor
                                                        • Epidermal growth factor Epidermal growth factor
                                                        • Fas Ligand Fas Ligand
                                                        • Granulocyte Macrophage Colony Stimulating Factor Granulocyte Macrophage Colony Stimulating Factor
                                                        • Leukemia Inhibitory Factor Leukemia Inhibitory Factor
                                                        • Luteinizing hormone Luteinizing hormone
                                                        • Macrophage Migration Inhibitory Factor Macrophage Migration Inhibitory Factor
                                                        • Interferon Beta Interferon Beta
                                                        • Interferon Gamma Interferon Gamma
                                                        • Transforming Growth Factor Beta 1 Transforming Growth Factor Beta 1
                                                        • B Type Natriuretic Peptide B Type Natriuretic Peptide
                                                        • Glial Fibrillary Acidic Protein Glial Fibrillary Acidic Protein
                                                        • Cystatin C Cystatin C
                                                        • Hepatocyte Growth Factor Hepatocyte Growth Factor
                                                        • Interferon Alpha 2 Interferon Alpha 2
                                                        • Tumor Necrosis Factor Alpha Tumor Necrosis Factor Alpha

                                                        Expertise

                                                        • Liquid Chromatography Mass Spectrometry
                                                        • ELISA Method
                                                        • MSD Assay
                                                        • Luminex Multiplex Assay
                                                        • LI-COR Odyssey Assay
                                                        • PK PD Analysis
                                                        • Method Development Validation and Transfer

                                                        Services

                                                        • Pharmacokinetics (PK) Study
                                                        • Toxicology (Tox) Study
                                                        • Biomarker Testing Services
                                                        • Bioequivalence and Bioavailability Studies
                                                        • Immunogenicity Testing
                                                        • Bioanalytical Assay

                                                        Solutions

                                                        • Bioanalysis
                                                        • GLP Laboratory
                                                        • Clinical CRO
                                                        • Preclinical CRO
                                                        • Analytical Testing
                                                        • Non-GLP Testing

                                                        About Us

                                                        • Overview
                                                        • Why Us?
                                                        • Values
                                                        • Leadership
                                                        • Process
                                                        • Quality and Compliance
                                                        • Facility and Equipment
                                                        • Therapeutic Areas
                                                        • Partners
                                                        • Careers

                                                        Resources

                                                        • Learning Center
                                                        • Blog
                                                        • Case Study
                                                        • News and Events
                                                        • Testimonials
                                                        • Contact Us
                                                        NorthEast BioLab Logo
                                                        925 Sherman Avenue Hamden, CT 06514
                                                        sales@nebiolab.com
                                                        (203) 361 3780

                                                        Consult with a Scientist Now

                                                        We Will Contact You Within 1 Business Day

                                                        • Good Laboratory Practice for Nonclinical Laboratory Studies
                                                        • Food and Drug Administration (FDA)
                                                        • United States Drug Enforcement Administration
                                                        • Connecticut State Department of Consumer Protection
                                                        • Privacy Policy
                                                        • Terms of Use
                                                        • Sitemap
                                                        © 2019 - 2025, NorthEast BioAnalytical Laboratories LLC. All rights reserved.

                                                        Before you go! Get
                                                        your FREE Download:

                                                        Top 7 Hidden Pitfalls to your Bioanalytical Milestones

                                                        Step by step guide on how to accelerate your drug approval

                                                        • Addressing Main Constraints
                                                        • Research Best Practices
                                                        • The Economic Advantage
                                                        • Critical Decision Marketing Mode Easy
                                                        • Legal Documentation Checklist
                                                        We won’t share your email, ever, See our privacy policy

                                                        No thanks, I don’t need to improve my drug approval process